PXD005786

PXD005786 is an original dataset announced via ProteomeXchange.

Title	Oncogenic BRD4-NUT protein interactions
Description	To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma, we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared to wild type BRD4. Using crosslinking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 among a small number of candidates associated with BRD4-NUT in NMC patient cells but not present in 293T cells.
HostingRepository	PRIDE
AnnounceDate	2017-04-06
AnnouncementXML	Submission_2017-05-11_06:28:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barry Zee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos; LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-26 02:06:44	ID requested
1	2017-04-05 05:04:03	announced
2	2017-04-06 00:30:22	announced	Updated project metadata.
⏵ 3	2017-05-11 06:28:22	announced	Updated project metadata.

Alekseyenko AA, Walsh EM, Zee BM, Pakozdi T, Hsi P, Lemieux ME, Dal Cin P, Ince TA, Kharchenko PV, Kuroda MI, French CA, Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma. Proc Natl Acad Sci U S A, 114(21):E4184-E4192(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: BioTAP-XL, hyperacetylation, ZNF532-NUT, topological domains

Mitzi I. Kuroda
contact affiliation	Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA
contact email	mkuroda@genetics.med.harvard.edu
lab head
Barry Zee
contact affiliation	Harvard Medical School
contact email	bzee@genetics.med.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005786

PRIDE project URI

• PRIDE
  1. PXD005786
     1. Label: PRIDE project
     2. Name: Oncogenic BRD4-NUT protein interactions