PXD005785

PXD005785 is an original dataset announced via ProteomeXchange.

Title	The effect of chronic inflammation on molecular pathways of long-term potentiation in an APP/PS1 mouse model of Alzheimer Disease
Description	Alzheimer Disease (AD) is the most common cause of dementia and neuroinflammation, initiated by systemic bacterial infection, is believed to contribute significantly to its pathogenesis. Lipopolysaccharide (LPS) is an endotoxin, which stimulates inflammatory reactions by increasing the levels of pro-inflammatory cytokines through binding to Toll like receptor-4 (TLR-4) and CD14, in an NF-κΒ-dependent fashion. These receptors are expressed in the circumventricular organs, choroid plexus and leptomeninges as well as on microglia cells, thereby providing an entry for an innate immune reaction to spread through the cerebral tissue. Here, 9 months old APP695/PS1ΔE9 (APP/PS1) double transgenic mice and wild type littermates were treated with weekly intraperitoneal injections of LPS or PBS over 13 weeks to evaluate the effect of repeated infections on the innate immune response of the hippocampal proteome. The effect on the proteome was evaluated using a quantitative iTRAQ 8-plex proteomics approach, with 3D-LC-MS/MS to gain a deep coverage of the hippocampal proteome. Furthermore, the effect of long-term inflammation on amyloid beta (Aβ) protein level was evaluated using stereological plaque load estimation followed by investigation of protein levels of Aβ40 and Aβ42 by Mesoscale. An effect of infection on proteins involved in long-term potentiation was observed, including p-MAPK, p-CREB and phosphorylation motifs of PKA and 14-3-3. Furthermore the complement system, redox reactions and the activation of retinoid receptors were found significantly changed in APP/PS1 mice infected with LPS. This study provides new insight into the effect of bacterial infections in the development of AD.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:48.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan J. Kempf
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-26 02:02:04	ID requested
1	2018-11-21 16:24:47	announced
2	2018-11-23 06:49:32	announced	Updated publication reference for PubMed record(s): 30459560.
⏵ 3	2024-10-22 04:35:50	announced	2024-10-22: Updated project metadata.

10.3389/fncel.2018.00397;

Thygesen C, Ilkj, æ, r L, Kempf SJ, Hemdrup AL, von Linstow CU, Babcock AA, Darvesh S, Larsen MR, Finsen B, Transgenic Mice Implicate Cathepsin Z in Alzheimer's Disease. Front Cell Neurosci, 12():397(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: neuroinflammation, brain, LTP,LPS

Martin R. Larsen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
contact email	mrl@bmb.sdu.dk
lab head
Stefan J. Kempf
contact affiliation	University of Southern Denmark
contact email	kempf-stefan@t-online.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD005785

PRIDE project URI

• PRIDE
  1. PXD005785
     1. Label: PRIDE project
     2. Name: The effect of chronic inflammation on molecular pathways of long-term potentiation in an APP/PS1 mouse model of Alzheimer Disease