PXD005780

PXD005780 is an original dataset announced via ProteomeXchange.

Title	Relating human gut metagenome and metaproteome
Description	Gut microbiome research is rapidly moving towards the functional characterization of the microbiota by means of shotgun meta-omics. Here, we selected a cohort of healthy subjects from an indigenous and monitored Sardinian population to analyze their gut microbiota using both shotgun metagenomics and shotgun metaproteomics. We found a considerable divergence between genetic potential and functional activity of the human healthy gut microbiota, in spite of a quite comparable taxonomic structure revealed by the two approaches. Investigation of inter-individual variability of taxonomic features revealed Bacteroides and Akkermansia as remarkably conserved and variable in abundance within the population, respectively. Firmicutes-driven butyrogenesis (mainly due to Faecalibacterium spp.) was shown to be the functional activity with the higher expression rate and the lower inter-individual variability in the study cohort, highlighting the key importance of the biosynthesis of this microbial by-product for the gut homeostasis. The taxon-specific contribution to functional activities and metabolic tasks was also examined, giving insights into the peculiar role of several gut microbiota members in carbohydrate metabolism (including polysaccharide degradation, glycan transport, glycolysis and short-chain fatty acid production). In conclusion, our results provide useful indications regarding the main functions actively exerted by the gut microbiota members of a healthy human cohort, and support metaproteomics as a valuable approach to investigate the functional role of the gut microbiota in health and disease.
HostingRepository	PRIDE
AnnounceDate	2017-07-19
AnnouncementXML	Submission_2017-07-19_00:21:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Tanca
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-25 02:35:20	ID requested
⏵ 1	2017-07-19 00:21:23	announced

Tanca A, Abbondio M, Palomba A, Fraumene C, Manghina V, Cucca F, Fiorillo E, Uzzau S, Potential and active functions in the gut microbiota of a healthy human cohort. Microbiome, 5(1):79(2017) [pubmed]

curator keyword: Metaproteomics, Biological

submitter keyword: gut metagenome, gut metaproteome, metaproteomics

Sergio Uzzau
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy
contact email	uzzau@portocontericerche.it
lab head
Alessandro Tanca
contact affiliation	Proteomics Laboratory, Porto Conte Ricerche, Alghero, Italy
contact email	tanca@portocontericerche.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD005780

PRIDE project URI

• PRIDE
  1. PXD005780
     1. Label: PRIDE project
     2. Name: Relating human gut metagenome and metaproteome