PXD005772

PXD005772 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MALDI Quant Fibrinogen
Description	Absolute quantification of protein was performed by MALDI-LTQ using scramble internal standard (sIS) peptide. Mixtures of serial dilutions of fibrinogen peptides (final concentration: 50, 100, 250, 500 and 1000 fmol/μL), equal amounts of corresponding sIS peptides (final concentration: 500 fmol/μL) and serial dilutions of digested serum (final concentration: 0, 0.025, 0.05, 0.25 and 0.5 μg/μL) were analyzed by MALDI-MS/MS. We also analyzed immunoprecipitated (IP) α-fibrinogen in 0.05-5 μL of plasma by MALDI-MS/MS.
HostingRepository	jPOST
AnnounceDate	2017-04-08
AnnouncementXML	Submission_2022-09-18_04:06:52.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumio Ohtsuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrument	MALDI LTQ XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-24 00:31:54	ID requested
1	2017-04-07 08:00:06	announced
2	2018-09-18 23:58:24	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 17:17:10	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 04:06:52	announced	2022-09-18: Updated FTP location.

Publication List

Yoneyama T, Ohtsuki S, Tachikawa M, Uchida Y, Terasaki T, Scrambled Internal Standard Method for High-Throughput Protein Quantification by Matrix-Assisted Laser Desorption Ionization Tandem Mass Spectrometry. J Proteome Res, 16(4):1556-1565(2017) [pubmed]

Yoneyama T, Ohtsuki S, Tachikawa M, Uchida Y, Terasaki T, Scrambled Internal Standard Method for High-Throughput Protein Quantification by Matrix-Assisted Laser Desorption Ionization Tandem Mass Spectrometry. J Proteome Res, 16(4):1556-1565(2017) [pubmed]

Keyword List

submitter keyword: MALDI Quant

submitter keyword: MALDI Quant

Contact List

Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliation	Kumamoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000231/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000231/