PXD005765

PXD005765 is an original dataset announced via ProteomeXchange.

Title	HLA-E Presents Glycopeptides from the Mtb Protein MPT32 to Human CD8 T Cells
Description	Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes Tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8+ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classical MHC I molecule HLA-E can present pathogen-derived peptides to CD8+ T cells. In this manuscript, we identified the antigen recognized by an HLA-E-restricted CD8+ T cell clone isolated from an Mtb latently infected individual as a peptide from the Mtb protein, MPT32. Recognition by the CD8+ T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene. This is the first description of a post-translationally modified Mtb-derived protein antigen presented in the context of an HLA-E specific CD8+ T cell immune response. The identification of an immune response that targets a unique mycobacterial modification is novel and has practical impact in the development of vaccines and diagnostics.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_10:21:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005765
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lisa Wolfe
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	Hex; Oxidation; Carbamidomethyl
Instrument	LTQ; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-23 02:40:34	ID requested
⏵ 1	2018-10-26 10:21:20	announced

Harriff MJ, Wolfe LM, Swarbrick G, Null M, Cansler ME, Canfield ET, Vogt T, Toren KG, Li W, Jackson M, Lewinsohn DA, Dobos KM, Lewinsohn DM, T cells. Sci Rep, 7(1):4622(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: HLA-E, Mycobacterium tuberculosis, CD8 T Cells

Karen Marie Dobos
contact affiliation	Colorado State University
contact email	lisa.wolfe@colostate.edu
lab head
Lisa Wolfe
contact affiliation	Colorado State University
contact email	lisa.wolfe@colostate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005765

PRIDE project URI

• PRIDE
  1. PXD005765
     1. Label: PRIDE project
     2. Name: HLA-E Presents Glycopeptides from the Mtb Protein MPT32 to Human CD8 T Cells