PXD005760

PXD005760 is an original dataset announced via ProteomeXchange.

Title	A Proteomic View of Escherichia coli Metabolic Features
Description	Escherichia coli is an important human pathogen, among others a cause of severe diarrhea diseases and urinary tract infections. The ability to distinguish different pathogenic E. coli subspecies is crucial for correct treatment of the infection. Characterization and quantification of clinical isolates proteomes can provide details of the organisms’ metabolism and specific virulence factors. We performed a systematic quantitative proteomic analysis on a representative selection of 16 pathogenic and 2 commensal E. coli strains, together with 5 pathogenic Shigella strains. The analysis yielded a dataset of more than 4 thousand proteins, with an average of 2 thousand proteins per strain and 980 proteins common to all strains. Statistical comparison of label-free quantitative levels of 750 proteins, which were quantified in all strains, revealed that levels of a majority of the shared proteins vary substantially among specific strains. Theses quantitative protein profiles clearly distinguished E. coli strains from Shigella and largely separated commensal E. coli strains from intestinal and extraintestinal E. coli isolates.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_01:17:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronika Kucharova
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-23 01:18:02	ID requested
⏵ 1	2020-10-19 01:17:15	announced

Pettersen VK, Steinsland H, Wiker HG, Distinct Metabolic Features of Pathogenic Escherichia coli and Shigella spp. Determined by Label-Free Quantitative Proteomics. Proteomics, 21(2):e2000072(2021) [pubmed]

curator keyword: Biological

submitter keyword: Escherichia coli, Label-Free Quantification, Bacterial Metabolism, Virulence

Harald G. Wiker
contact affiliation	The Gade Research Group for Infection and Immunity, Department of Clinical Science, University of Bergen, Norway
contact email	Harald.Wiker@uib.no
lab head
Veronika Kucharova
contact affiliation	University of Bergen
contact email	veronika.kucharova@k2.uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD005760

PRIDE project URI

• PRIDE
  1. PXD005760
     1. Label: PRIDE project
     2. Name: A Proteomic View of Escherichia coli Metabolic Features