PXD005753

PXD005753 is an original dataset announced via ProteomeXchange.

Title	PTMs of HTT protein
Description	Post-translational modifications (PTMs) of proteins regulate various cellular processes. PTMs of polyglutamine-expanded huntingtin (Htt) protein, causative of Huntington’s disease (HD), are likely modulators of HD pathogenesis. Previous studies have identified and characterized several PTMs on exogenously expressed Htt fragments, however none of these studies were designed to systematically characterize PTMs on the endogenous full-length Htt protein.We found that full-length endogenous Htt, immunoprecipitated from HD knock-in mouse and human post mortem brain, is suitable for detection of PTMs by mass spectrometry. Using label-free mass spectrometry, we identified around 40 PTMs, of which half are novel. These findings will be instrumental in the further assembling the Htt PTM framework, and validate PTMs of Htt as promising therapeutic targets for HD.
HostingRepository	PRIDE
AnnounceDate	2017-06-29
AnnouncementXML	Submission_2017-06-29_06:19:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005753
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tamara Ratovitski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	HNE; Hex; CAMthiopropanoyl; CarbamidomethylDTT; GlycerylPE; Propionamide; BADGE; Deamidated; sulfo+amino; Trp->Oxolactone; CHDH; Glucuronyl; Cation:K; NO_SMX_SMCT; Gln->pyro-Glu; Met->Hsl; Diethylphosphate; DAET; DeStreak; BHTOH; NA-LNO2; Trimethyl; Ammonia-loss; Ethylphosphate; Oxidation; AEC-MAEC; Carboxy; BITC; O-pinacolylmethylphosphonate; 4-ONE; PET; Ethanolamine; Cation:Na; PyMIC; Trp->Hydroxykynurenin; GlyGly; Deoxy; Pyro-carbamidomethyl; Formyl; Carbamyl; LG-anhydrolactam; Cation:Mg[II]; Thiophospho; Tyr->Dha; Ammonium; 2-succinyl; Cation:Cu[I]; Crotonaldehyde; Cytopiloyne; Dethiomethyl; Amidated; Thioacyl; Cys->Dha; Pro->pyro-Glu; Phospho; Hep; Cation:Zn[II]; PEITC; Acetyl; Galactosyl; Nitro; Methylthio; Carboxymethyl; HexNAc; Carbamidomethyl; 3sulfo; Cation:Ca[II]; Trioxidation; trifluoro; Glu->pyro-Glu; CarboxymethylDTT; MercaptoEthanol; Hex(1)HexNAc(1)NeuAc(1); EDT-maleimide-PEO-biotin; dHex; Delta:H(2)C(2); Ethyl; Cation:Fe[II]; O-Isopropylmethylphosphonate; Cyano; Methyl; AEBS; Pyridylacetyl; Methylmalonylation; Glucosylgalactosyl; Pyridylethyl; HNE-Delta:H(2)O; Didehydro; Cytopiloyne+water; Trp->Kynurenin; SMA; Iminobiotin; Iodo; Cys->Oxoalanine; SecCarbamidomethyl; Hydroxycinnamyl; Lys->Allysine; LG-anhyropyrrole; Amidine; Hex(1)HexNAc(1)NeuAc(2); Dehydrated; Biotin; Dioxidation; Benzoyl; glycidamide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-20 04:32:18	ID requested
1	2017-06-29 06:14:48	announced
⏵ 2	2017-06-29 06:19:47	announced	Updated publication reference for PubMed record(s): 2865385, 28653853.

Ando S, [Biochemistry of brain aging]. Nihon Rinsho, 43(7):1399-403(1985) [pubmed]

Ratovitski T, O'Meally RN, Jiang M, Chaerkady R, Chighladze E, Stewart JC, Wang X, Arbez N, Roby E, Alexandris A, Duan W, Vijayvargia R, Seong IS, Lavery DJ, Cole RN, Ross CA, Post-Translational Modifications (PTMs), Identified on Endogenous Huntingtin, Cluster within Proteolytic Domains between HEAT Repeats. J Proteome Res, 16(8):2692-2708(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Huntingtin, HD, human and mouse brain

Christopher A. Ross
contact affiliation	Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, US
contact email	caross@jhu.edu
lab head
Tamara Ratovitski
contact affiliation	JHU
contact email	tratovi1@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD005753

PRIDE project URI

• PRIDE
  1. PXD005753
     1. Label: PRIDE project
     2. Name: PTMs of HTT protein