PXD005748

PXD005748 is an original dataset announced via ProteomeXchange.

Title	A quantitative proteomic profile revealed the crucial roles of lysosome in the microbial infection
Description	Lysosome played vital roles for both innate and adaptive immunity. It has been widely accepted that lysosome functioned more than a digestive organelle. However, it was still largely unknown for the proteome changes of lysosome on various microbes, and the understanding of lysosomal proteome from the purified lysosome is another obstacle that needs to be resolved. Here, we performed a proteomic study on the enriched lysosomes from THP1 cells after infected by Listeria monocytogenes (L. m), Herpes Simplex Virus 1 (HSV-1) and Vesicular Stomatitis Virus (VSV). Combining with the GO analysis, we identified 284 lysosomal related proteins from the totally identified 4560 proteins. We also constructed the protein-protein interaction networks for the changed proteins and revealed the core lysosomal proteins in responding for diverse microbial infections. From this study, we not only revealed that lysosome responded in a different manner for the bacterial or virus infection, but also proposed a new way for the proteome research for the enriched organelles.
HostingRepository	PRIDE
AnnounceDate	2017-05-04
AnnouncementXML	Submission_2017-05-04_06:14:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-20 02:19:10	ID requested
⏵ 1	2017-05-04 06:14:30	announced

Xu B, Gao Y, Zhan S, Ge W, Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections. Mol Immunol, 87():122-131(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Lysosome, monocyte, proteomics, hypoxia

Wei Ge
contact affiliation	State Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005748
     1. Label: PRIDE project
     2. Name: A quantitative proteomic profile revealed the crucial roles of lysosome in the microbial infection