PXD005747

PXD005747 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome analysis of bone marrow-derived dendritic cells infected by Francisella tularensis
Description	Francisella tularensis is a highly infective Gram-negative bacterium, also known as the causative agent of tularemia. The disease is characterized by the delayed onset of the adaptive immune response which provides time for the bacterial replication. As an intracellular pathogen, Francisella proliferates mainly in the cytosol of host phagocytes, which are linkers between the innate and the adaptive immunity. In particular, dendritic cells (DCs) are known to be the most effective antigen-presenting cells and thus they are crucial for the induction of the adaptive response. However, Francisella is able to invade and to proliferate inside DCs while avoiding their effective activation and maturation. The goal of this study was to map phosphoproteome changes in DCs infected by Francisella in order to reveal host signaling pathways involved in Francisella-DC interaction. The focus was laid on early intervals (<1 h p.i.) because of the ability of Francisella to escape in this time period from the phagosome to the cytosol where it establishes its replicative niche. For comparison, attenuated and in vivo-protective Francisella strain was used in parallel in experiments. DC phosphoproteome was analyzed by the means of shotgun LC-MS-based proteomics and relative changes were quantified by the stable isotope labeling method specially developed for primary bone marrow-derived DCs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:38.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Fabrik
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Francisella tularensis subsp. holarctica FSC200; NCBI TaxID: 351581;
ModificationList	6x(13)C labeled L-lysine; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-20 02:11:31	ID requested
1	2017-10-26 04:29:24	announced
⏵ 2	2024-10-22 04:35:42	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra117.000160;

Fabrik I, Link M, Putzova D, Plzakova L, Lubovska Z, Philimonenko V, Pavkova I, Rehulka P, Krocova Z, Hozak P, Santic M, Stulik J, Strain Occurs in Phases and Involves the Activation of Extracellular Signal-Regulated Kinases (ERKs) and p38 In the Later Stage. Mol Cell Proteomics, 17(1):81-94(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Francisella tularensis,Bone marrow-derived dendritic cells, MAPK signaling, Phosphoproteome

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Ivo Fabrik
contact affiliation	Biomedical Research Center, University Hospital Hradec Kralove
contact email	ivo.fabrik@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD005747

PRIDE project URI

• PRIDE
  1. PXD005747
     1. Label: PRIDE project
     2. Name: Phosphoproteome analysis of bone marrow-derived dendritic cells infected by Francisella tularensis