HCT116 or K562 cells treated with either DMSO or E7820 were analyzed with data independent acquisition (DIA).&#10;The protein alkylation and digestion procedure followed the Filter Aided Sample Preparation (FASP) method, with some modification. Tryptic peptides from the whole cell lysate or immunoprecipitation samples were analyzed in a nano-flow LC-MS/MS system using a Q Exactive™ HF mass spectrometer (Thermo Fisher Scientific) coupled with an online UltiMate(R) 3000 Rapid Separation LC (RSLC, Dionex) and an HTC PAL sample injector (CTC Analytics, Zwingen, Switzerland) fitted with a microcapillary column (360 nm outer diameter (OD) × 100 μm ID), which was packed with < 20 cm of ReproSil C18-AQ 5 μm beads (Dr. Maisch GmbH) and equipped with an integrated electrospray emitter tip (P-2000 laser-based puller; Sutter Instruments).