PXD005742

PXD005742 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mode of action of anticancer sulfonamides
Description	HCT116 or K562 cells treated with either DMSO or E7820 were analyzed with data independent acquisition (DIA). The protein alkylation and digestion procedure followed the Filter Aided Sample Preparation (FASP) method, with some modification. Tryptic peptides from the whole cell lysate or immunoprecipitation samples were analyzed in a nano-flow LC-MS/MS system using a Q Exactive™ HF mass spectrometer (Thermo Fisher Scientific) coupled with an online UltiMate(R) 3000 Rapid Separation LC (RSLC, Dionex) and an HTC PAL sample injector (CTC Analytics, Zwingen, Switzerland) fitted with a microcapillary column (360 nm outer diameter (OD) × 100 μm ID), which was packed with < 20 cm of ReproSil C18-AQ 5 μm beads (Dr. Maisch GmbH) and equipped with an integrated electrospray emitter tip (P-2000 laser-based puller; Sutter Instruments).
HostingRepository	jPOST
AnnounceDate	2017-01-19
AnnouncementXML	Submission_2022-09-18_02:37:07.813.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taisuke Uehara
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; N6-acetyl-L-lysine; alpha-amino acetylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-18 17:39:50	ID requested
1	2017-01-18 17:39:54	announced
2	2018-09-18 23:59:20	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 16:50:22	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:37:08	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: HCT116, K562

submitter keyword: HCT116, K562

Contact List

Taisuke Uehara
lab head
Taisuke Uehara
contact affiliation	Eisai Co., Ltd.
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000232/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000232/