PXD005731

PXD005731 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	BHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells
Description	Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, DTMUV can also infect geese, chickens, house sparrows and replicate in in the brain, spleen, liver and kidneys of BALB/c mice, which poses public health concerns. So developing vaccines to combat DTMUV becomes urgent. Baby hamster kidney cell line (BHK-21) is usually employed to produce veterinary vaccine and DTMUV as well as other flaviviruses can be propagated in BHK-21 cells. However, information about mammalian host cell responses to DTMUV infection is limited. In this study, LC–MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells. We identified 192 differentially expressed cellular proteins including 11 upregulated proteins and 8 downregulated at 24 hpostinfection; 25 upregulated proteins and 151 downregulated at 48 h postinfection. Some of these proteins were involved in viral infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:43.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Sun
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-17 03:20:54	ID requested
1	2017-05-23 00:21:43	announced
⏵ 2	2024-10-22 04:35:48	announced	2024-10-22: Updated project metadata.

Publication List

10.1002/pmic.201700033;
Sun X, Wang S, Lin X, Zhao L, Zhang D, Yi C, Sun X, Chen H, Jin M, Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics, 17(12):(2017) [pubmed]

10.1002/pmic.201700033;

Sun X, Wang S, Lin X, Zhao L, Zhang D, Yi C, Sun X, Chen H, Jin M, Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics, 17(12):(2017) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: DTMUV infection, iTRAQ,BHK-21 cell

curator keyword: Technical

submitter keyword: DTMUV infection, iTRAQ,BHK-21 cell

Contact List

Meilin Jin
contact affiliation	State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, China
contact email	jml8328@126.com
lab head
Xin Sun
contact affiliation	Huazhong Agricultual University
contact email	coolxin@foxmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005731
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005731

PRIDE project URI

Repository Record List

[ + ]