PXD005731 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells |
Description | Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, DTMUV can also infect geese, chickens, house sparrows and replicate in in the brain, spleen, liver and kidneys of BALB/c mice, which poses public health concerns. So developing vaccines to combat DTMUV becomes urgent. Baby hamster kidney cell line (BHK-21) is usually employed to produce veterinary vaccine and DTMUV as well as other flaviviruses can be propagated in BHK-21 cells. However, information about mammalian host cell responses to DTMUV infection is limited. In this study, LC–MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells. We identified 192 differentially expressed cellular proteins including 11 upregulated proteins and 8 downregulated at 24 hpostinfection; 25 upregulated proteins and 151 downregulated at 48 h postinfection. Some of these proteins were involved in viral infection. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:35:43.803.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xin Sun |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-01-17 03:20:54 | ID requested | |
1 | 2017-05-23 00:21:43 | announced | |
⏵ 2 | 2024-10-22 04:35:48 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/pmic.201700033; |
Sun X, Wang S, Lin X, Zhao L, Zhang D, Yi C, Sun X, Chen H, Jin M, Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics, 17(12):(2017) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: DTMUV infection, iTRAQ,BHK-21 cell |
Contact List
Meilin Jin |
contact affiliation | State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, China |
contact email | jml8328@126.com |
lab head | |
Xin Sun |
contact affiliation | Huazhong Agricultual University |
contact email | coolxin@foxmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005731 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005731
- Label: PRIDE project
- Name: BHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells