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PXD005731

PXD005731 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells
DescriptionDuck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, DTMUV can also infect geese, chickens, house sparrows and replicate in in the brain, spleen, liver and kidneys of BALB/c mice, which poses public health concerns. So developing vaccines to combat DTMUV becomes urgent. Baby hamster kidney cell line (BHK-21) is usually employed to produce veterinary vaccine and DTMUV as well as other flaviviruses can be propagated in BHK-21 cells. However, information about mammalian host cell responses to DTMUV infection is limited. In this study, LC–MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells. We identified 192 differentially expressed cellular proteins including 11 upregulated proteins and 8 downregulated at 24 hpostinfection; 25 upregulated proteins and 151 downregulated at 48 h postinfection. Some of these proteins were involved in viral infection.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:35:43.803.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXin Sun
SpeciesList scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-01-17 03:20:54ID requested
12017-05-23 00:21:43announced
22024-10-22 04:35:48announced2024-10-22: Updated project metadata.
Publication List
10.1002/pmic.201700033;
Sun X, Wang S, Lin X, Zhao L, Zhang D, Yi C, Sun X, Chen H, Jin M, Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics, 17(12):(2017) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: DTMUV infection, iTRAQ,BHK-21 cell
Contact List
Meilin Jin
contact affiliationState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, China
contact emailjml8328@126.com
lab head
Xin Sun
contact affiliationHuazhong Agricultual University
contact emailcoolxin@foxmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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