PXD005724

PXD005724 is an original dataset announced via ProteomeXchange.

Title	Phagolysosomal proteomics identifies different macrophage activation profiles in response to Aspergillus fumigatus conidia
Description	An Aspergillus fumigatus infection is initialized by the inhalation and germination of airborne asexual spores (conidia). Alveolar macrophages are the first immune cells to counteract an infection by phagocytosis of conidia and intracellular degradation of the pathogen. However, A. fumigatus circumvents its intracellular killing by the manipulation of the phagolysosomal maturation. With a comparative proteomic study of the phagolysosomal proteome of a virulent wild type A. fumigatus strain and an avirulent mutant strain we aimed at the identification of proteins and processes that are hijacked by the virulent strain. We found that wild type conidia strongly modulate the protein composition of the phagolysosome. Assembly and activity of vATPase, formation of lipid rafts, signal transduction pathways as well as the generation of energy and metabolites were significantly regulated in the wild type conidia containing phagolysosomes. In different experimental setups we confirmed a disassembly of the vATPase complex, reduced formation of lipid rafts and altered abundances of mTOR, MAPK signaling molecules, Rab5 and Vamp8 mediators of endosomal trafficking as well as LAMP1 and cathepsin Z lysosomal markers. Our interactome analysis indicates that A. fumigatus proteins target small GTPases of the endosomal trafficking system and signal transducers of the host, which might lead to a reduced fungicidal activity of the phagolysosome.
HostingRepository	PRIDE
AnnounceDate	2018-03-08
AnnouncementXML	Submission_2018-03-08_02:44:41.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005724
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationList	residues isobaric at 113.084064 Da; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-17 00:12:31	ID requested
⏵ 1	2018-03-08 02:44:42	announced

Schmidt H, Vlaic S, Kr, ü, ger T, Schmidt F, Balkenhol J, Dandekar T, Guthke R, Kniemeyer O, Heinekamp T, Brakhage AA, Conidia-containing Phagolysosomes Identifies Processes Governing Immune Evasion. Mol Cell Proteomics, 17(6):1084-1096(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Aspergillus fumigatus, phagolysosome, macrophages, host-pathogen interaction

Axel A. Brakhage
contact affiliation	Department of Molecular and Applied Microbiology, Leibniz Institute of Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Adolf-Reichwein-Str. 23, D-07745 Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005724
     1. Label: PRIDE project
     2. Name: Phagolysosomal proteomics identifies different macrophage activation profiles in response to Aspergillus fumigatus conidia