PXD005717

PXD005717 is an original dataset announced via ProteomeXchange.

Title	Integrin beta 3 regulates cellular senescence by activating the TGFβ pathway
Description	Cellular senescence is an important in vivo mechanism that prevents the propagation of damaged cells. However, the precise mechanism(s) regulating senescence are not well characterized. Here, we find that ITGB3 (integrin beta 3 or β3) is epigenetically regulated by the Polycomb protein CBX7. β3 expression accelerates the onset of senescence in human primary fibroblasts, by activating the TGFβ pathway in a cell autonomous and non-cell autonomous manner. β3 levels are dynamically increased during oncogeneinduced senescence (OIS) through CBX7 epigenetic regulation. DNA-damage and therapy-induced senescence (TIS) also induce β3 expression. In fact, downregulation of β3 levels override OIS and TIS, independently of its ligand-binding activity. Moreover, cilengitide, a αvβ3 antagonist, has the ability to block the SASP without affecting proliferation. Finally, we show an increase in β3 levels during aging in mice and humans. Altogether, our data show that integrin β3 subunit is a marker and regulator of senescence.
HostingRepository	PRIDE
AnnounceDate	2017-03-09
AnnouncementXML	Submission_2017-06-05_08:23:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vesela Encheva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-16 08:04:03	ID requested
1	2017-03-09 03:45:44	announced
⏵ 2	2017-06-05 08:23:37	announced	Updated publication reference for PubMed record(s): 28273461.

Rapisarda V, Borghesan M, Miguela V, Encheva V, Snijders AP, Lujambio A, O'Loghlen A, Pathway. Cell Rep, 18(10):2480-2493(2017) [pubmed]

curator keyword: Biological

submitter keyword: senescence, SILAC, CBX7

Ambrosius Snijders
contact affiliation	Bram Snijders Head of Mass Spectrometry Proteomics Platform The Francis Crick Institute 1 Midland Road London NW1 1AT T: +442037962038 E: bram.snijders@crick.ac.uk W: https://crims.crick.ac.uk/
contact email	Bram.Snijders@crick.ac.uk
lab head
Vesela Encheva
contact affiliation	The Francis Crick Institute
contact email	Vesela.Encheva@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005717

PRIDE project URI

• PRIDE
  1. PXD005717
     1. Label: PRIDE project
     2. Name: Integrin beta 3 regulates cellular senescence by activating the TGFβ pathway