PXD005708

PXD005708 is an original dataset announced via ProteomeXchange.

Title	N-Glycoproteomic Analysis of Botryococcus Braunii
Description	We have performed an N-glycoproteomic analysis of the green microalgae Botryococcus braunii, a promising candidate for the production of biofuels, accumulating considerable amounts of hydrocarbon oils. Thereby, three different strains have been compared: Showa (Race B), AC761 (Race B) and CCALA778 (Race A) which differ in the type of produced hydrocarbons. In total, 517 unique N-glycosylated peptides have been identified analyzing intact N-glycopeptides as well as deglycosylated, 18O-labeled peptides. Intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the non-reducing end were identified. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. This type of methylated GnTI-dependent N-glycans has not been described so far.
HostingRepository	PRIDE
AnnounceDate	2017-08-08
AnnouncementXML	Submission_2017-08-08_12:51:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Botryococcus braunii; NCBI TaxID: 38881;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-16 01:30:59	ID requested
⏵ 1	2017-08-08 12:51:48	announced

Schulze S, Urzica E, Reijnders MJMF, van de Geest H, Warris S, Bakker LV, Fufezan C, Martins Dos Santos VAP, Schaap PJ, Peters SA, Hippler M, Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni. New Phytol, 215(4):1361-1369(2017) [pubmed]

curator keyword: Biological

submitter keyword: Botryococcus braunii, N-glycosylation, algae

Michael Hippler
contact affiliation	Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany
contact email	mhippler@uni-muenster.de
lab head
Stefan Schulze
contact affiliation	University of Muenster
contact email	stefan.schulze@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005708
     1. Label: PRIDE project
     2. Name: N-Glycoproteomic Analysis of Botryococcus Braunii