PXD005699

PXD005699 is an original dataset announced via ProteomeXchange.

Title	Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger
Description	Renewables-based biotechnology depends on enzymes to degrade plant lignocellulose to simple sugars that are converted to fuels or high-value products. Identification and characterization of such lignocellulose degradative enzymes could be fast-tracked by availability of an enzyme activity measurement method that is fast, label-free, uses minimal resources and allows direct identification of generated products. We developed such a method by applying carbohydrate arrays coupled with MALDI-ToF mass spectrometry to identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Aspergillus niger. We describe the production and characterization of plant polysaccharide-derived oligosaccharides and their attachment to hydrophobic self-assembling monolayers on a gold target. We verify effectiveness of this array for detecting exo- and endo-acting glycoside hydrolase activity using commercial enzymes, and demonstrate how this platform is suitable for detection of enzyme activity in relevant biological samples, the culture filtrate of A. niger grown on wheat straw. In conclusion, this versatile method is broadly applicable in screening and characterisation of activity of CAZymes, such as fungal enzymes for plant lignocellulose degradation with relevance to biotechnological applications as biofuel production, the food and animal feed industry.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_10:35:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005699
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jolanda van Munster
SpeciesList	scientific name: Aspergillus niger; NCBI TaxID: 5061;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-12 03:20:20	ID requested
⏵ 1	2018-10-26 10:35:57	announced

van Munster JM, Thomas B, Riese M, Davis AL, Gray CJ, Archer DB, Flitsch SL, Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger. Sci Rep, 7():43117(2017) [pubmed]

curator keyword: Technical

submitter keyword: Aspergillus niger, plant-polysaccharide degrading enzymes, CAZymes, lignocellulose

David Archer
contact affiliation	University of Nottingham, School of Life Sciences
contact email	David.Archer@Nottingham.ac.uk
lab head
Jolanda van Munster
contact affiliation	University of Manchester
contact email	Jolandavanmunster@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005699
     1. Label: PRIDE project
     2. Name: Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger