PXD005688

PXD005688 is an original dataset announced via ProteomeXchange.

Title	Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections
Description	Neutrophils have an important role in rapid antimicrobial defenses against and resolution of urinary tract infections (UTIs). We show that a mechanism known as neutrophil extracellular trap (NET) formation is a strategy to combat pathogens in the urinary tract. Viscous aggregates that were present in human urine sediments revealed high extracellular DNA content. The DNA formed a scaffold to which antimicrobial effector proteins derived from different neutrophil subcellular compartments bound. Treatment with deoxyribonuclease I solubilized these structures. We observed massive proteolytic degradation in the NETs with apparently dominant roles for the neutrophil granule proteases elastase, proteinase 3, and cathepsin G. In a UTI case with Staphylococcus aureus as the infectious agent, high abundance of autolysins and immune evasion factors in a cell-free NET extract suggested that controlled cell wall autolysis plays a role in derailing the host immune response and allows some bacterial cells to survive following entrapment in NETs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:51.439.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanbao Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-11 02:36:36	ID requested
1	2017-01-24 01:18:49	announced
2	2017-02-02 05:56:49	announced	Updated publication reference for PubMed record(s): 28129394.
⏵ 3	2024-10-22 04:34:59	announced	2024-10-22: Updated project metadata.

10.1371/journal.ppat.1006151;

Yu Y, Kwon K, Tsitrin T, Bekele S, Sikorski P, Nelson KE, Pieper R, Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections. PLoS Pathog, 13(1):e1006151(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Urine, Neutrophil Extracellular Traps (NETs), Urinary Tract Infection (UTI), Proteomics, LCMSMS

Yanbao Yu
contact affiliation	J. Craig Venter Institute, Rockville MD 20850 USA
contact email	yayu@jcvi.org
lab head
Yanbao Yu
contact affiliation	University of Delaware
contact email	yayu@jcvi.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005688

PRIDE project URI

• PRIDE
  1. PXD005688
     1. Label: PRIDE project
     2. Name: Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections