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PXD005687

PXD005687 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMycobacterium tuberculosis ON-A strain (Wild type vs natural mutant)
DescriptionTuberculosis remains one of the most difficult to control infectious diseases in the world. Many different factors contribute to the complexity of this disease. These include the ability of the host to control the infection which may directly relate to nutritional status, presence of co-morbidities and genetic predisposition. Pathogen factors, in particular the ability of different Mycobacterium tuberculosis strains to respond to the harsh environment of the host granuloma, which includes low oxygen and nutrient availability and the presence of damaging radical oxygen and nitrogen species, also play an important role in the success of different strains to cause disease. In this study we evaluate the impact of a naturally occurring 12 gene 15 Kb genomic deletion on the physiology and virulence of M. tuberculosis. The strains denominated ON-A WT (wild type) and ON-A NM (natural mutant) were isolated from a previously reported TB outbreak in an inner city under-housed population in Toronto, Canada. Here we subjected these isogenic strains to transcriptomic (via RNA-seq) and proteomic analyses and identified several gene clusters with differential expression in the natural mutant, including the DosR regulon and the molybdenum cofactor biosynthesis genes, both of which were found in lower abundance in the natural mutant. We also demonstrated lesser virulence of the natural mutant in the guinea pig animal model. Overall, our findings suggest that the ON-A natural mutant is less fit to cause disease, supporting the general idea that even low virulent strains have the potential to cause extended transmission in at risk populations.
HostingRepositoryPRIDE
AnnounceDate2018-04-23
AnnouncementXMLSubmission_2018-04-23_05:10:28.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005687
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCarolina Mehaffy
SpeciesList scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-01-11 01:39:37ID requested
12018-04-23 05:10:30announced
Publication List
Duncan C, Jamieson FB, Troudt J, Izzo L, Bielefeldt-Ohmann H, Izzo A, Mehaffy C, Whole transcriptomic and proteomic analyses of an isogenic M. tuberculosis clinical strain with a naturally occurring 15 Kb genomic deletion. PLoS One, 12(6):e0179996(2017) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Mycobacterium tuberculosis
Contact List
Carolina Mehaffy
contact affiliationDepartment of Microbiology, Immunology and Pathology; Colorado State University; Fort Collins, CO, USA
contact emailcarolina.mehaffy@colostate.edu
lab head
Carolina Mehaffy
contact affiliationColorado State University
contact emailcarolina.mehaffy@colostate.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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