PXD005666

PXD005666 is an original dataset announced via ProteomeXchange.

Title	IL-22 Controls Iron-Dependent Nutritional Immunity Against Systemic Bacterial Infections
Description	Host immunity limits iron availability to pathogenic bacteria, but whether immunity limits pathogenic bacteria from accessing host heme, the major source of iron in the body, remains unclear. Using Citrobacter rodentium, a mouse enteric pathogen and Escherichia coli, a major cause of sepsis in humans as models, we find that interleukin-22, a cytokine best known for its ability to promote epithelial barrier function, also suppresses the systemic growth of bacteria by limiting iron availability to the pathogen. Using an unbiased proteomic approach to understand the mechanistic basis of IL-22 dependent iron retention in the host, we have identified that IL-22 induces the production of the plasma hemoglobin scavenger haptoglobin and heme scavenger hemopexin. Moreover, the anti-microbial effect of IL-22 depends on the induction of hemopexin expression, while haptogloblin is dispensable. Impaired pathogen clearance in infected Il22-/- mice was restored by hemopexin administration and hemopexin-deficient mice had increased pathogen loads after infection. These studies reveal a previously unrecognized host defense mechanism regulated by IL-22 that relies on the induction of hemopexin to limit heme availability to bacteria leading to suppression of bacterial growth during systemic infections.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:40.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Nunez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-09 01:33:43	ID requested
1	2017-02-03 00:47:42	announced
2	2017-06-05 08:37:34	announced	Updated publication reference for PubMed record(s): 28286877.
⏵ 3	2024-10-22 04:34:42	announced	2024-10-22: Updated project metadata.

10.1126/sciimmunol.aai8371;

Sakamoto K, Kim YG, Hara H, Kamada N, Caballero-Flores G, Tolosano E, Soares MP, Puente JL, Inohara N, N, ú, ñ, ez G, IL-22 Controls Iron-Dependent Nutritional Immunity Against Systemic Bacterial Infections. Sci Immunol, 2(8):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: hemopexin, IL-22, bacterial infection

Gabriel Nunez
contact affiliation	Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
contact email	bclx@med.umich.edu
lab head
Gabriel Nunez
contact affiliation	University of Michigan
contact email	bclx@med.umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005666

PRIDE project URI

• PRIDE
  1. PXD005666
     1. Label: PRIDE project
     2. Name: IL-22 Controls Iron-Dependent Nutritional Immunity Against Systemic Bacterial Infections