PXD005658

PXD005658 is an original dataset announced via ProteomeXchange.

Title	HIV-1 activates T cell signaling independently of antigen to drive viral spread
Description	HIV-1 spreads between CD4 T cells most efficiently through virus-induced cell-cell contacts. To test whether this process potentiates viral spread by activating signaling pathways we developed an approach to analyze the phosphoproteome in infected and uninfected mixed population T cells using differential metabolic labeling and mass spectrometry. We discovered HIV-1 induced activation of signaling networks during viral spread encompassing over 200 cellular proteins. Strikingly, pathways downstream of T cell receptor were the most significantly activated, despite the absence of canonical antigen dependent stimulation. The importance of this pathway was demonstrated by depletion of proteins and we show that HIV-1 Env mediated cell-cell contact, T cell receptor and the Src kinase Lck were essential for signaling dependent enhancement of viral dissemination. This study demonstrates that manipulation of signaling at immune cell contacts by HIV-1 is essential for promoting virus replication and defines a new paradigm for antigen independent T cell signaling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:04.582.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clare Jolly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-05 01:17:27	ID requested
1	2022-02-28 03:04:29	announced
⏵ 2	2024-10-22 05:34:05	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2016.12.057;

Len ACL, Starling S, Shivkumar M, Jolly C, HIV-1 Activates T Cell Signaling Independently of Antigen to Drive Viral Spread. Cell Rep, 18(4):1062-1074(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: T cell,HIV, phosphorylation, virological synapse

Clare Jolly
contact affiliation	Division of Infection and Immunity University College London London United Kingdom
contact email	c.jolly@ucl.ac.uk
lab head
Clare Jolly
contact affiliation	University College London
contact email	c.jolly@ucl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD005658

PRIDE project URI

• PRIDE
  1. PXD005658
     1. Label: PRIDE project
     2. Name: HIV-1 activates T cell signaling independently of antigen to drive viral spread