PXD005657

PXD005657 is an original dataset announced via ProteomeXchange.

Title	Direct evidence of the presence of cross-linked Aβ dimers in the brains of Alzheimer’s disease patients
Description	Brain-derived amyloid-β (Aβ) dimers are associated with Alzheimer´s disease (AD). However, their covalent nature remains controversial. This feature is relevant, as a covalent cross-link would make brain-derived dimers (native dimers) more synaptotoxic than Aβ monomers and would make them suitable candidates for biomarker development. To resolve this controversy, we here present a three-step approach. First, we validated a type of synthetic cross-linked Aβ (CL Aβ) dimers, obtained by means of the photo-induced cross-linking of unmodified proteins (PICUP) reaction, as well-defined mimics of putative native CL Aβ dimers. Second, we used these PICUP CL Aβ dimers as standards to improve the isolation of native Aβ dimers and to develop state-of-the-art mass spectrometry (MS) strategies to allow their characterization. Third, we applied these MS methods to the analysis of native Aβ dimer samples allowing the detection of the CL [Aβ(6-16)]2 peptide comprising a dityrosine cross-link. This result demonstrates the presence of CL Aβ dimers in the brains of patients with AD and opens up avenues for establishing new therapeutic targets and developing novel biomarkers for this disease.
HostingRepository	PRIDE
AnnounceDate	2021-05-13
AnnouncementXML	Submission_2021-05-13_03:35:16.574.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Gay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-04 06:02:02	ID requested
⏵ 1	2021-05-13 03:35:17	announced

V, á, zquez de la Torre A, Gay M, Vilapriny, ó, -Pascual S, Mazzucato R, Serra-Batiste M, Vilaseca M, Carulla N, Dimers in the Brains of Alzheimer's Disease Patients. Anal Chem, 90(7):4552-4560(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Alzheimer’s disease, amyloid-β, cross-linked dimers, oxidative stress

Natàlia Carulla
contact affiliation	Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 10, 08028 Barcelona, Spain CBMN (UMR 5248), University of Bordeaux – CNRS – IPB, Institut Européen de Chimie et Biologie, 2 rue Escarpit, 33600, Pessac, France
contact email	n.carulla@iecb.u-bordeaux.fr
lab head
Marina Gay
contact affiliation	Institute for Research in Biomedicine
contact email	marina.gay@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD005657

PRIDE project URI

• PRIDE
  1. PXD005657
     1. Label: PRIDE project
     2. Name: Direct evidence of the presence of cross-linked Aβ dimers in the brains of Alzheimer’s disease patients