PXD005654

PXD005654 is an original dataset announced via ProteomeXchange.

Title	MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting
Description	Develeopment and application of MS Western method. A new GeLC-MS/MS method is developed for the absolute quantification of proteins.
HostingRepository	PRIDE
AnnounceDate	2017-12-18
AnnouncementXML	Submission_2017-12-18_00:38:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005654
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mukesh Kumar
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Label:13C(6)15N(4); Oxidation; Label:13C(6); Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-03 23:30:50	ID requested
⏵ 1	2017-12-18 00:38:28	announced

Kumar M, Joseph SR, Augsburg M, Bogdanova A, Drechsel D, Vastenhouw NL, Buchholz F, Gentzel M, Shevchenko A, MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting. Mol Cell Proteomics, 17(2):384-396(2018) [pubmed]

curator keyword: Technical

submitter keyword: GeLC-MS/MS, Absolute Quantification

Andrej Shevchenko
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
contact email	shevchenko@mpi-cbg.de
lab head
Mukesh Kumar
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
contact email	kumar@mpi-cbg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD005654

PRIDE project URI

• PRIDE
  1. PXD005654
     1. Label: PRIDE project
     2. Name: MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting