PXD005647

PXD005647 is an original dataset announced via ProteomeXchange.

Title	Deciphering the Protein-Protein Interaction Network Regulating Hepatocelluler Carcinoma Metastasis
Description	Hepatocellular carcinoma (HCC) is one of the leading causes of mortality related to cancer all over the world. The poor prognosis of HCC is mostly due to recurrence and tumor metastasis. In order to better understand the molecular mechanisms of HCC metastasis, we analyzed the proteome of three HCC cell lines with different metastasis potentials by using quantitative proteomics and bioinformatics analysis. As a result, we identified 331 cellular proteins potentially associated to HCC metastasis, and constructed a highly connected protein-protein interaction (PPI) network. Functional annotation of the network uncovered prominent pathways and key roles of these proteins, suggesting that metabolism and cytoskeleton biological progresses are greatly involved with HCC metastasis. Furthermore, integrative network analysis revealed a rich-club organization in the PPI network indicating a hub center of connections, including several well-known cancer related proteins, such as SRC proto-oncogene, non-receptor tyrosine kinase (SRC) and pyruvate kinase M2 (PKM2). Moreover, the differential expressions of two identified proteins, including PKM2 and actin-related protein 2/3 complex subunit 4 (ARPC4), were validated using Western blotting. These two proteins were identified as potential prognostic markers for HCC by using survival rate analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:47.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruibing Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-03 03:56:00	ID requested
1	2017-06-20 04:15:11	announced
2	2018-01-30 06:29:24	announced	Updated publication reference for PubMed record(s): 29371291, 28627476.
⏵ 3	2024-10-22 04:34:59	announced	2024-10-22: Updated project metadata.

10.1016/j.bbapap.2017.06.005;

10.1074/mcp.ra118.000586;

Li Y, Zhuang H, Zhang X, Li Y, Liu Y, Yi X, Qin G, Wei W, Chen R, Multiomics Integration Reveals the Landscape of Prometastasis Metabolism in Hepatocellular Carcinoma. Mol Cell Proteomics, 17(4):607-618(2018) [pubmed]

Qin G, Dang M, Gao H, Wang H, Luo F, Chen R, Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. Biochim Biophys Acta Proteins Proteom, 1865(9):1114-1122(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Dimethylation

HCC

LC-MS/MS

Protein-Protein Interaction

Quantitative Proteomics

Tumor Progression

Chen Ruibing
contact affiliation	Tianjin Medical University
contact email	chenruibing@tijmu.edu.cn
lab head
Ruibing Chen
contact affiliation	Tianjin Medical University
contact email	chenruibing@tijmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD005647

PRIDE project URI

• PRIDE
  1. PXD005647
     1. Label: PRIDE project
     2. Name: Deciphering the Protein-Protein Interaction Network Regulating Hepatocelluler Carcinoma Metastasis