PXD005646

PXD005646 is an original dataset announced via ProteomeXchange.

Title	SILAC-based Quantitative Proteomic Analysis Unveiled the Arsenite-induced Perturbation of Multiple Pathways in Human Skin Fibroblast Cells
Description	Humans are exposed to arsenic species through inhalation, ingestion and dermal contact, which may lead to skin, liver, and bladder cancers as well as cardiovascular and neurological diseases. The mechanisms underlying the cytotoxic and carcinogenic effects of arsenic species, however, remain incompletely understood. To exploit the mechanisms of toxicity of As(III), we employed stable isotope labeling by amino acids in cell culture (SILAC) together with LC-MS/MS analysis to assess quantitatively the As(III)-induced perturbation of the entire proteome of cultured human skin fibroblast cells. Shotgun proteomic analysis on an LTQ-Orbitrap Velos mass spectrometer facilitated the quantification of more than 3800 proteins, 250 of which displayed significant alternations (> 1.5 fold) upon arsenite treatment. Targeted analysis on a triple-quadrupole mass spectrometer in the multiple-reaction monitoring (MRM) mode confirmed the quantification results of some select proteins. Ingenuity pathway analysis revealed the arsenite-induced alteration of more than 10 biological pathways, including the Nrf2-mediated oxidative stress response pathway as reflected by the up-regulation of 9 proteins in this pathway. In addition, arsenite induced changes in expression levels of a number of selenoproteins and metallothioneins. Together, the results from the present study painted a more complete picture about the biological pathways that are altered in human skin fibroblast cells upon arsenite exposure.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_11:19:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fan Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-03 03:44:30	ID requested
⏵ 1	2018-10-26 11:19:13	announced

Zhang F, Xiao Y, Wang Y, SILAC-Based Quantitative Proteomic Analysis Unveils Arsenite-Induced Perturbation of Multiple Pathways in Human Skin Fibroblast Cells. Chem Res Toxicol, 30(4):1006-1014(2017) [pubmed]

submitter keyword: arsenite, oxidative stress, quantitative proteomics, mass spectrometry, SILAC

Fan Zhang
contact affiliation	University of California Riverside
contact email	fanzhang330@gmail.com
lab head
Fan Zhang
contact affiliation	University of California Riverside
contact email	fanzhang330@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005646

PRIDE project URI

• PRIDE
  1. PXD005646
     1. Label: PRIDE project
     2. Name: SILAC-based Quantitative Proteomic Analysis Unveiled the Arsenite-induced Perturbation of Multiple Pathways in Human Skin Fibroblast Cells