PXD005635

PXD005635 is an original dataset announced via ProteomeXchange.

Title	Detecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters
Description	Homogeneous gold nanoclusters (Au NCs) have been widely utilized in drug delivery, chemical sensing, bioassays and bio-labeling due to their unique physical and chemical properties. However, few attentions have been paid on their application in detecting protein post-translational modifications. Herein, we developed a homogeneous reaction system with water-soluble zwitterionic Au NCs to capture glycopeptides from the complex biological samples for the first time. The unique characteristics of Au NCs, such as the molecular-like property, the excellent homogeneity in aqueous solution and organic solvent responsive precipitation, the easy preparation in only 4.5 hours, contribute to the high efficiency and high through-put for capturing the targeted glycopeptides. Briefly, the Au NCs and tryptic protein samples were incubated together in a homogeneous aqueous solution and then the glycopeptides were collected by co-precipitation with zwitterionic Au NCs dur-ing gradually dropping addition of acetonitrile. Compared with the conventional heterogeneous system with solid-state ad-sorbents, the number of characterized glycosylation sites was improved 35%. Finally, the MS detection limitation as low as 50 amol was achieved for the tryptic digests of standard glycoprotein (IgG) and 1576 glycosylation sites from 713 glycopro-teins were feasibly identified from only 60 μg mouse liver proteins
HostingRepository	PRIDE
AnnounceDate	2017-03-29
AnnouncementXML	Submission_2017-03-29_00:53:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinan Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-03 01:12:19	ID requested
⏵ 1	2017-03-29 00:53:24	announced

Li J, Liu J, Liu Z, Tan Y, Liu X, Wang F, Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters. Anal Chem, 89(8):4339-4343(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: gold cluster, glycopeptides, homogeneous reaction system

Fangjun Wang
contact affiliation	Dalian Institute of Chemical Physics
contact email	wangfj@dicp.ac.cn
lab head
Jinan Li
contact affiliation	Dalian Institute of Chemical Physics
contact email	lijinan0824@dicp.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005635
     1. Label: PRIDE project
     2. Name: Detecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters