PXD005633

PXD005633 is an original dataset announced via ProteomeXchange.

Title	Hrd1 interaction proteomics - Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation
Description	Endoplasmic reticulum (ER)-associated degradation (ERAD) mediates the degradation of misfolded and unoligomerized proteins in the early secretory pathway. ERAD substrates are detected and delivered to membrane-embedded dislocation complexes. Following transfer into the cytosol, substrates are rapidly ubiquitinated and targeted to the 26S proteasome for degradation. Hrd1 is a highly conserved, ER-resident E3 ubiquitin-protein ligase that functions in ERAD. In this study, we employed stable isotope labeling with amino acids in cell culture (SILAC) to quantitatively assess the impact of altered lipid homeostasis on the composition of S-tagged Hrd1 complexes affinity purified from HEK293 cells. Although lipid disequilibrium impaired ERAD substrate delivery to Hrd1, the overall composition of the Hrd1 complex was unaffected.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:15.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Olzmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	6410 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-02 07:42:40	ID requested
1	2017-01-02 23:58:19	announced
2	2017-01-03 00:30:14	announced	Updated project metadata.
3	2017-10-24 06:04:45	announced	Updated project metadata.
⏵ 4	2024-10-22 04:35:23	announced	2024-10-22: Updated project metadata.

To M, Peterson CW, Roberts MA, Counihan JL, Wu TT, Forster MS, Nomura DK, Olzmann JA, Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation. Mol Biol Cell, 28(2):270-284(2017) [pubmed]

curator keyword: Biological

submitter keyword: ERAD, Hrd1, triacsin C

James Arthur Olzmann
contact affiliation	Department of Nutritional Sciences and Toxicology University of California, Berkeley
contact email	olzmann@berkeley.edu
lab head
James Olzmann
contact affiliation	University of California, Berkeley
contact email	jolzmann@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005633

PRIDE project URI

• PRIDE
  1. PXD005633
     1. Label: PRIDE project
     2. Name: Hrd1 interaction proteomics - Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation