PXD005628

PXD005628 is an original dataset announced via ProteomeXchange.

Title	CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs
Description	Mitochondria are essential organelles that host crucial metabolic pathways and produce ATP. The mitochondrial proteome is heterogeneous among tissues, and can dynamically change in response to different metabolic conditions. While the transcriptional programs that govern mitochondrial biogenesis and respiratory function are well-known, post-transcriptional regulatory mechanisms remain unclear. Here we show that the cytosolic RNA-binding protein CLUH regulates the expression of a mitochondrial protein network supporting key metabolic programs required under nutrient deprivation. CLUH exerts its function by controlling the stability and translation of target mRNAs. In absence of Cluh, mitochondria are severely depleted of crucial enzymes involved in catabolic energy-converting pathways. CLUH preserves oxidative mitochondrial function and glucose homeostasis, thus preventing death at the foetal-neonatal transition. In the adult liver, CLUH ensures maximal respiration capacity and the metabolic response to starvation. Our results shed new light into post-transcriptional mechanisms controlling the expression of mitochondrial proteins, and suggest novel strategies to tailor mitochondrial function to physiological and pathological conditions.
HostingRepository	PRIDE
AnnounceDate	2017-02-08
AnnouncementXML	Submission_2017-06-06_08:16:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-02 02:57:13	ID requested
1	2017-02-08 00:15:28	announced
⏵ 2	2017-06-06 08:16:20	announced	Updated publication reference for PubMed record(s): 28188211.

Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI, CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol, 216(3):675-693(2017) [pubmed]

curator keyword: Biological

submitter keyword: SILAC mouse, post transcriptional gene expression control

Christian K. Frese
contact affiliation	Head Proteomics Core Facility University of Cologne CECAD Research Center Joseph-Stelzmann-Str. 26 50931 Cologne
contact email	christian.frese@uni-koeln.de
lab head
Christian Frese
contact affiliation	CECAD Research Center Cologne
contact email	cfrese@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005628
     1. Label: PRIDE project
     2. Name: CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs