PXD005625

PXD005625 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema
Description	Paladin (Pald1, mKIAA1274 or x99384) was identified in screens for vascular-specific genes and is a putative phosphatase. We have demonstrated that paladin has a predominant vascular expression pattern and shifts from endothelial to mural cells during mouse development. We have now characterized the Pald1 knock-out mouse in a broad array of behavioral, physiological and biochemical tests. Here, we show that female, but not male, Pald1 heterozygous and homozygous knock-out mice displayed an emphysema-like phenotype with increased alveolar air spaces and impaired lung function with obstructive changes. In contrast to many other tissues where Pald1 is restricted to the vascular compartment, Pald1 is expressed in both the epithelial and mesenchymal compartments of the postnatal lung. However, in Pald1 knock-out females, there is a specific increase in apoptosis and proliferation of endothelial cells, but not in non-endothelial cells. This results in a transient reduction of endothelial cells in the maturing lung. Our data suggests that paladin is required during lung vascular development and for normal function of the developing and adult lung in a sex-specific manner. To our knowledge, this is the first report of a sex-specific effect on endothelial cell apoptosis. Proteomic analysis of Pald1 wild type and knock-out mice reveal that most of the protein expression differences are related to sex and not genotype, supporting the notion that sex can be a major factor for lung development and disease. In addition, Pald1 wild type and knock-out mice exhibit differential expression of HPGD, hydroxyprostaglandin dehydrogenase 15 (NAD), the major enzyme for degradation of prostaglandins.
HostingRepository	jPOST
AnnounceDate	2017-11-01
AnnouncementXML	Submission_2022-09-18_02:22:05.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik Johansson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-22 06:07:22	ID requested
1	2017-10-31 08:00:06	announced
2	2018-09-10 06:23:48	announced	Updated PubMed.
3	2018-09-18 23:52:18	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:02:04	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 02:22:06	announced	2022-09-18: Updated FTP location.

Publication List

Ega, ñ, a I, Kaito H, Nitzsche A, Becker L, Ballester-Lopez C, Niaudet C, Petkova M, Liu W, Vanlandewijck M, Vernaleken A, Klopstock T, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Rask-Andersen H, Johansson HJ, Lehti, ö J, He L, Yildirim A Ö, Hellstr, ö, m M, Aguilar-Pimentel A, Ollert M, Schmidt-Weber C, Amarie O, Graw J, Beckers J, Garrett L, H, ö, lter SM, Zimprich A, Wurst W, Moreth K, Bekeredjian R, Neff F, Calzada-Wack J, R, á, cz I, Zimmer A, Rathkolb B, Wolf E, Rozman J, Klingenspor M, Stoeger T, Eickelberg O, Treise I, Busch DH, Ö, stereicher M, Steinkamp R, Lengger C, Maier H, Stoeger C, Leuchtenberger S, Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema. Sci Rep, 7(1):15453(2017) [pubmed]

Ega, ñ, a I, Kaito H, Nitzsche A, Becker L, Ballester-Lopez C, Niaudet C, Petkova M, Liu W, Vanlandewijck M, Vernaleken A, Klopstock T, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Rask-Andersen H, Johansson HJ, Lehti, ö J, He L, Yildirim A Ö, Hellstr, ö, m M, Aguilar-Pimentel A, Ollert M, Schmidt-Weber C, Amarie O, Graw J, Beckers J, Garrett L, H, ö, lter SM, Zimprich A, Wurst W, Moreth K, Bekeredjian R, Neff F, Calzada-Wack J, R, á, cz I, Zimmer A, Rathkolb B, Wolf E, Rozman J, Klingenspor M, Stoeger T, Eickelberg O, Treise I, Busch DH, Ö, stereicher M, Steinkamp R, Lengger C, Maier H, Stoeger C, Leuchtenberger S, Female mice lacking Pald1 exhibit endothelial cell apoptosis and emphysema. Sci Rep, 7(1):15453(2017) [pubmed]

Keyword List

submitter keyword: Pald1, knock-out, lung, emphysema, Hpgd

submitter keyword: Pald1, knock-out, lung, emphysema, Hpgd

Contact List

Janne Lehtiö
lab head
Henrik Johansson
contact affiliation	Karolinska Institutet
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000225/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000225/