PXD005616

PXD005616 is an original dataset announced via ProteomeXchange.

Title	Matrisome remodelling during Intervertebral Disc development and ageing
Description	Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM remodelling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was used to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder samples and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:08.120.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005616
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	4700 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-21 02:37:46	ID requested
1	2017-09-25 02:18:58	announced
⏵ 2	2024-10-22 04:35:16	announced	2024-10-22: Updated project metadata.

10.6019/PXD005616;

10.1038/s41598-017-11960-0;

Caldeira J, Santa C, Os, ó, rio H, Molinos M, Manadas B, Gon, ç, alves R, Barbosa M, Matrisome Profiling During Intervertebral Disc Development And Ageing. Sci Rep, 7(1):11629(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Foetus,Extracellular matrix, Development, Intervertebral Disc, Ageing

Hugo Osorio
contact affiliation	i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto Ipatimup - Institute of Molecular Pathology and Immunology of the University of Porto Rua Alfredo Allen, 208 4200-135 Porto Portugal
contact email	hosorio@ipatimup.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health of the University of Porto / Ipatimup - Institute of Molecular Pathology and Immunology of the University of Porto
contact email	hosorio@ipatimup.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005616

PRIDE project URI

• PRIDE
  1. PXD005616
     1. Label: PRIDE project
     2. Name: Matrisome remodelling during Intervertebral Disc development and ageing