PXD005612

PXD005612 is an original dataset announced via ProteomeXchange.

Title	Capturig the Asc1p/RACK1 microenviroment at the head region of the 40S ribosome with quantitative BioID
Description	The Asc1 protein of Saccharomyces cerevisiae is a Gβ-like scaffold protein considered to organize the local microenvironment at the head region of ribosomes in order to link mRNA translation with cellular signaling. In this study we used the in vivo protein labeling technique proximity-dependent Biotin IDentification (BioID) in combination with stable isotope labeling with amino acids in cell culture (SILAC) in S. cerevisiae to quantitatively monitor the proteinaceous Asc1p neighborhood in exponentially growing cells, but also to record alterations in response to glucose deprivation and heat stress. Beyond expected known interaction partners several proteins not known to co-localize with Asc1p were identified including mRNA-binding proteins, translational regulators and even transcription-related proteins. Additionally, we observed major alterations in response to glucose withdrawal. In contrast, heat stress caused only minor effects on the Asc1p neighborhood. Furthermore, we analyzed the molecular microenvironment of the “ribosome-binding deficient” Asc1DE protein, which revealed ribosome-association of the major Asc1DEp population in vivo, but suggests a distorted position of the variant at the ribosome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:00.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	biotinylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-21 01:54:49	ID requested
1	2017-10-09 03:07:02	announced
⏵ 2	2024-10-22 04:38:01	announced	2024-10-22: Updated project metadata.

Opitz N, Schmitt K, Hofer-Pretz V, Neumann B, Krebber H, Braus GH, Valerius O, (RACK1) Microenvironment at the Head Region of the 40S Ribosome with Quantitative BioID in Yeast. Mol Cell Proteomics, 16(12):2199-2218(2017) [pubmed]

10.1074/mcp.m116.066654;

curator keyword: Biological

submitter keyword: LC-MSMS, Asc1p, RACK1, SILAC, signal transduction,Saccharomyces cerevisiae, BioID, translation

Dr. Oliver Valerius
contact affiliation	Georg-August-University Göttingen Department of Molecular Microbiology and Genetics Institute of Microbiology and Genetics
contact email	ovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005612
     1. Label: PRIDE project
     2. Name: Capturig the Asc1p/RACK1 microenviroment at the head region of the 40S ribosome with quantitative BioID