PXD005610

PXD005610 is an original dataset announced via ProteomeXchange.

Title	Label free quantitative mass spectrometry identifies platelet degranulation as an early event during platelet storage
Description	Shelf life of platelet concentrates (PCs) is limited to up to 7 days due to loss of platelet functionality during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify changes in the platelet proteome during storage. In total 2501 proteins were accurately quantified in 3 biological replicates on at least 1 of the 7 different time-points analyzed. Significant changes in levels of 21 proteins were observed over time. Gene ontology (GO) enrichment analysis of these proteins revealed that the majority of this set was involved in platelet degranulation, secretion and regulated exocytosis. Twelve of these proteins, which included SPARC and von Willebrand factor (VWF), have been shown to reside in α-granules; a significant decline in levels of these proteins was already observed at day 5 of storage. A strongly reduced level of Protein S100A9 (S100A9) which is predominantly localized in the cytoplasm was observed at day 5 of storage. We also monitored changes upon prolonged storage (13-16 days); identifying elevated levels of α-2-macroglobulin, glycogenin and Ig μ chain C region. The ability of platelets to bind and/or endocytose α-2-macroglobulin was confirmed by confocal microscopy. Taken together, our data show that release of α-granules is an early event during platelet storage. In addition, we show that platelets can bind or ingest proteins during storage which may have impact on the hemostatic properties of platelets used for transfusion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:11.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maaike Rijkers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-21 01:40:44	ID requested
1	2017-09-11 02:32:13	announced
⏵ 2	2024-10-22 04:35:17	announced	2024-10-22: Updated project metadata.

10.1038/s41598-017-11643-w;

Rijkers M, van den Eshof BL, van der Meer PF, van Alphen FPJ, de Korte D, Leebeek FWG, Meijer AB, Voorberg J, Jansen AJG, Monitoring storage induced changes in the platelet proteome employing label free quantitative mass spectrometry. Sci Rep, 7(1):11045(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: proteomics, Label Free Quantification, alpha-granules, platelet storage,Platelets

Jan Voorberg
contact affiliation	Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands
contact email	j.voorberg@sanquin.nl
lab head
Maaike Rijkers
contact affiliation	Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands
contact email	m.rijkers@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005610

PRIDE project URI

• PRIDE
  1. PXD005610
     1. Label: PRIDE project
     2. Name: Label free quantitative mass spectrometry identifies platelet degranulation as an early event during platelet storage