PXD005605

PXD005605 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of cell cycle arrest and differentiation induction caused by ATPR, a derivative of all-trans retinoic acid, in human gastric cancer SGC-7901 cells
Description	Experimental design: Peptides digested from the total cellular proteins were analyzed by reverse phase LC–MS/MS followed by a label-free quantification analysis. The SEQUEST search engine was used to identify proteins and bioinformatics resources were used to investigate the involved pathways for the differentially expressed proteins. Results: 13 down-regulated proteins were identified in the ATPR-treated group. Bioinformatics analysis showed that the effects of ATPR on 14-3-3 might potentially involve the PI3K-AKT-FOXO pathway and P27Kip1 expression. Western blot and RT-PCR analysis showed that ATPR could inhibit AKT phosphorylation, up-regulate the expression of FOXO1A and P27Kip1 at both the protein and mRNA levels, and down-regulate the cytoplasmic expression of cyclin E and CDK2. ATPR-induced G0/G1 phase arrest and differentiation can be ablated if the P27kip1 gene is silenced with sequence-specific siRNA. Conclusions and clinical relevance: ATPR might cause cell cycle arrest and differentiation in SGC-7901 cells by simultaneously inhibiting the phosphorylation of AKT and down-regulating 14-3-3. This change would then enhance the inhibition of cyclin E/CDK2 by up-regulating FOXO1A and P27Kip1. Our findings could be of value for finding new drug targets and for developing more effective differentiation inducer.
HostingRepository	PRIDE
AnnounceDate	2017-02-13
AnnouncementXML	Submission_2017-02-13_01:49:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xia Quan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-20 04:26:08	ID requested
⏵ 1	2017-02-13 01:49:49	announced

Xia Q, Zhao Y, Wang J, Qiao W, Zhang D, Yin H, Xu D, Chen F, Proteomic analysis of cell cycle arrest and differentiation induction caused by ATPR, a derivative of all-trans retinoic acid, in human gastric cancer SGC-7901 cells. Proteomics Clin Appl, 11(7-8):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: ATPR，Cell cycle arrest and differentiation，Proteomics analysis，PI3K-AKT-FOXO-P27kip1 pathway，SGC-7901 cells

Quan Xia
contact affiliation	School of Pharmacy, Anhui Medical University, Hefei, China
contact email	xiaquan2010@163.com
lab head
Xia Quan
contact affiliation	Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University
contact email	xiaquan2010@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005605
     1. Label: PRIDE project
     2. Name: Proteomic analysis of cell cycle arrest and differentiation induction caused by ATPR, a derivative of all-trans retinoic acid, in human gastric cancer SGC-7901 cells