PXD005601

PXD005601 is an original dataset announced via ProteomeXchange.

Title	mRNA-binding proteins of Leishmania donovani LC-MSMS
Description	Leishmania are unicellular eukaryotes responsible for leishmaniasis in humans. Like other trypanosomatids, leishmania regulate protein coding gene expression almost exclusively at the post-transcriptional level with the help of RNA binding proteins (RBPs). Due to the presence of polycystronic transcription units, leishmania do not regulate RNA polymerase II-dependent transcription initiation. Recent evidence suggests that the main control points in gene expression are mRNA degradation and translation. Protein-RNA interactions are involved in every aspect of RNA biology, such as mRNA splicing, polyadenylation, localization, degradation, and translation. A detailed picture of these interactions would likely prove to be highly informative in understanding leishmania biology and virulence. We developed a strategy involving covalent UV cross-linking of RBPs to mRNA in vivo, followed by interactome capture using oligo(dT) magnetic beads to define comprehensively the mRNA interactome of growing L. donovani amastigotes. The protein mass spectrometry analysis of captured proteins identified 81 mRNA interacting proteins which withstood very stringent washing conditions. Strikingly, we found that 49 of these mRNA interacting proteins had no orthologs or homologs in the human genome. Consequently, these may represent high quality candidates for selective drug targeting leading to novel therapeutics. These results show that this unbiased, systematic strategy has the promise to be applicable to study the mRNA interactome during various biological settings such as metabolic changes, stress (low pH environment, oxidative stress and nutrient deprivation) or drug treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:14.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Leishmania donovani; NCBI TaxID: 5661;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Bruker software

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-20 01:49:51	ID requested
1	2017-02-08 08:31:41	announced
⏵ 2	2024-10-22 04:35:22	announced	2024-10-22: Updated project metadata.

Nandan D, Thomas SA, Nguyen A, Moon KM, Foster LJ, Reiner NE, Comprehensive Identification of mRNA-Binding Proteins of Leishmania donovani by Interactome Capture. PLoS One, 12(1):e0170068(2017) [pubmed]

10.1371/journal.pone.0170068;

curator keyword: Biological, Biomedical

submitter keyword: LC-MSMS, mRNA-binding proteins,Leishmania donovani, interactome capture

Leonard James Foster
contact affiliation	Biochemistry and Molecular Biology, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005601

PRIDE project URI

• PRIDE
  1. PXD005601
     1. Label: PRIDE project
     2. Name: mRNA-binding proteins of Leishmania donovani LC-MSMS