PXD005600

PXD005600 is an original dataset announced via ProteomeXchange.

Title	Identification of new Stn7/Stn8 kinase targets reveals crosstalk between electron transport, carbohydrate metabolism and gene expression
Description	The thylakoid-associated kinases STN7 and STN8 are involved in short- and long-term acclimation of photosynthetic electron transport to changing light conditions. While the short term-response is well understood, the mechanistic details of the long-term response and the factors involved are enigmatic until now. Here we report the identification of new STN7/STN8 in vivo targets that connect photosynthetic electron transport with the regulation of metabolism and gene expression. Comparative phosphoproteomics with the stn7 and stn8 single and double mutants identified two proteases, one RNA-binding protein, a ribosomal protein, the large subunit of rubisco and a ferredoxin-NADP reductase as new targets for the thylakoid-associated kinases. Phosphorylation of three of the above proteins can be partially complemented by STN8 in the stn7 single mutant albeit at lower efficiency, while phosphorylation of the remaining three proteins strictly depends on STN7. The properties of the STN7-dependent phosphorylation site are similar to those of phosphorylated light-harvesting complex proteins entailing glycine or another small hydrophobic amino acid in the -1 position. Our analysis uncovers a so far unknown regulatory connection between photosynthetic electron transport, its immediate downstream sinks and long–term adaptation processes affecting metabolite accumulation and gene expression that is mediated by the STN7/STN8 kinase pair.
HostingRepository	PRIDE
AnnounceDate	2019-07-17
AnnouncementXML	Submission_2019-07-17_07:52:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sacha Baginsky
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-20 01:49:19	ID requested
⏵ 1	2019-07-17 07:52:15	announced

Sch, ö, nberg A, R, ö, diger A, Mehwald W, Galonska J, Christ G, Helm S, Thieme D, Majovsky P, Hoehenwarter W, Baginsky S, Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression. Plant J, 90(6):1176-1186(2017) [pubmed]

curator keyword: Biological

submitter keyword: shotgun proteomics, phosphoproteomics, kinase mutant, chloroplast

Sacha Baginsky
contact affiliation	Institut fuer Biochemie und Biotechnologie, Abteilung Pflanzenbiochemie
contact email	pflanzen@biochemtech.uni-halle.de
lab head
Sacha Baginsky
contact affiliation	Institut fuer Biochemie und Biotechnologie
contact email	pflanzen@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005600
     1. Label: PRIDE project
     2. Name: Identification of new Stn7/Stn8 kinase targets reveals crosstalk between electron transport, carbohydrate metabolism and gene expression