PXD005599

PXD005599 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome analysis of petunia corolla treated with ethylene and air
Description	Senescence represents the last stage of flower development. Phosphorylation is one of the key post-translational modifications that regulate protein functions in diverse biological pathways and contexts. Generally, kinases may be more required than phosphatases during plant growth and development. However, little is known about global phosphorylation change during flower senescence. In this work, we quantitatively investigated the petunia phosphoproteome following ethylene or air treatment. In total, 2170 phosphosites in 1184 protein groups were identified, among which 2059 sites in 1124 proteins were quantified. Treatment with ethylene resulted in 711 down-regulated and only 117 up-regulated phosphosites using a 1.5-fold threshold (P<0.05), showing that ethylene negatively regulates global phosphorylation levels and that phosphorylation of lots of proteins was not necessary during flower senescence. Our results show that protein dephosphorylation may play an important role of in ethylene-induced corolla senescence in petunia and that phosphatases may be more required than kinases during flower senescence. In addition, our results show that ethylene regulates ethylene and ABA signaling transduction pathways via phosphorylation level, and plant mRNA splicing machinery was a major target of ethylene-induced dephosphorylation. Moreover, ethylene treatment increases the number of alternative splicing of precursor RNAs in petunia corollas.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_13:13:01.184.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixun Yu
SpeciesList	scientific name: Petunia x hybrida; NCBI TaxID: 4102;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-20 01:44:11	ID requested
⏵ 1	2022-02-15 13:13:02	announced

Zhong S, Sang L, Zhao Z, Deng Y, Liu H, Yu Y, Liu J, Phosphoproteome analysis reveals the involvement of protein dephosphorylation in ethylene-induced corolla senescence in petunia. BMC Plant Biol, 21(1):512(2021) [pubmed]

submitter keyword: Phosphoproteome

petunia

ethylene

flower senescence

dephosphorylation

Yixun Yu
contact affiliation	College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	yuyixun@scau.edu.cn
lab head
Yixun Yu
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	yuyixun@scau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD005599

PRIDE project URI

• PRIDE
  1. PXD005599
     1. Label: PRIDE project
     2. Name: Phosphoproteome analysis of petunia corolla treated with ethylene and air