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PXD005598

PXD005598 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA fatty acid oxidation-dependent metabolic shift regulates adult neural stem cell quiescence
DescriptionAdult hippocampal neurogenesis is important for certain forms of cognition and failing neurogenesis has been implicated in neuropsychiatric diseases. The neurogenic capacity of hippocampal neural stem/progenitor cells (NSPCs) depends on a balance between quiescent and proliferative states. However, how this balance is regulated remains poorly understood. Here we show that the rate of fatty acid oxidation (FAO) defines quiescence vs. proliferation in NSPCs. Quiescent NSPCs show high levels of carnitine palmitoyltransferase 1a (Cpt1a)-dependent FAO, which is downregulated in proliferating NSPCs. Pharmacological inhibition and conditional deletion of Cpt1a in vitro and in vivo leads to altered NSPC behavior, showing that Cpt1a-dependent FAO is required for stem cell maintenance and proper neurogenesis. Strikingly, experimental manipulation of malonyl-CoA, the metabolite that regulates levels of FAO, is sufficient to induce exit from quiescence and to enhance NSPC proliferation. Thus, the data presented here identify a shift in FAO metabolism that governs NSPC behavior and suggest an instructive role for fatty acid metabolism in regulating NSPC activity.
HostingRepositoryPRIDE
AnnounceDate2019-11-15
AnnouncementXMLSubmission_2019-11-15_04:10:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Wegleiter
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListN-ethylmaleimide derivatized cysteine; hydrolyzed N-ethylmaleimide cysteine adduct; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-12-20 00:33:41ID requested
12019-11-15 04:10:15announced
Publication List
Knobloch M, Pilz GA, Ghesquière B, Kovacs WJ, Wegleiter T, Moore DL, Hruzova M, Zamboni N, Carmeliet P, Jessberger S, A Fatty Acid Oxidation-Dependent Metabolic Shift Regulates Adult Neural Stem Cell Activity. Cell Rep, 20(9):2144-2155(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: fatty acid oxidation, quiescence, neural stem cell
Contact List
Sebastian Jessberger
contact affiliationLaboratory of Neural Plasticity, Faculty of Medicine and Science, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland
contact emailjessberger@hifo.uzh.ch
lab head
Thomas Wegleiter
contact affiliationUniversity of Zurich
contact emailwegleiter@hifo.uzh.ch
dataset submitter
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