PXD005598 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A fatty acid oxidation-dependent metabolic shift regulates adult neural stem cell quiescence |
Description | Adult hippocampal neurogenesis is important for certain forms of cognition and failing neurogenesis has been implicated in neuropsychiatric diseases. The neurogenic capacity of hippocampal neural stem/progenitor cells (NSPCs) depends on a balance between quiescent and proliferative states. However, how this balance is regulated remains poorly understood. Here we show that the rate of fatty acid oxidation (FAO) defines quiescence vs. proliferation in NSPCs. Quiescent NSPCs show high levels of carnitine palmitoyltransferase 1a (Cpt1a)-dependent FAO, which is downregulated in proliferating NSPCs. Pharmacological inhibition and conditional deletion of Cpt1a in vitro and in vivo leads to altered NSPC behavior, showing that Cpt1a-dependent FAO is required for stem cell maintenance and proper neurogenesis. Strikingly, experimental manipulation of malonyl-CoA, the metabolite that regulates levels of FAO, is sufficient to induce exit from quiescence and to enhance NSPC proliferation. Thus, the data presented here identify a shift in FAO metabolism that governs NSPC behavior and suggest an instructive role for fatty acid metabolism in regulating NSPC activity. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-15 |
AnnouncementXML | Submission_2019-11-15_04:10:14.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Wegleiter |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | N-ethylmaleimide derivatized cysteine; hydrolyzed N-ethylmaleimide cysteine adduct; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-12-20 00:33:41 | ID requested | |
⏵ 1 | 2019-11-15 04:10:15 | announced | |
Publication List
Knobloch M, Pilz GA, Ghesqui, รจ, re B, Kovacs WJ, Wegleiter T, Moore DL, Hruzova M, Zamboni N, Carmeliet P, Jessberger S, A Fatty Acid Oxidation-Dependent Metabolic Shift Regulates Adult Neural Stem Cell Activity. Cell Rep, 20(9):2144-2155(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: fatty acid oxidation, quiescence, neural stem cell |
Contact List
Sebastian Jessberger |
contact affiliation | Laboratory of Neural Plasticity, Faculty of Medicine and Science, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland |
contact email | jessberger@hifo.uzh.ch |
lab head | |
Thomas Wegleiter |
contact affiliation | University of Zurich |
contact email | wegleiter@hifo.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD005598 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005598
- Label: PRIDE project
- Name: A fatty acid oxidation-dependent metabolic shift regulates adult neural stem cell quiescence