PXD005589

PXD005589 is an original dataset announced via ProteomeXchange.

Title	Dessert banana vs plantain: characterization of allele specific protein isoforms that are correlated to the fruit maturation process
Description	Dessert bananas have a soft texture and sweet taste at maturity. Plantain fruits belongs to Musa spp (AAB) and are known cooking bananas. This popular name is due to the fact that these fruits do keep a high amount of starch even after complete maturation and have a different flesh texture being not pleasant for fresh consumption. Plantains are a main starch food resource in African countries and India. Although a vital food source for big communities, studies about plantain are still scarce. The exploration of the biodiversity of the Musa gender can improve the breeding programs for many different problems, besides of a deeper knowledge about fruit development. We used here an easy and reproducible protocol for protein extraction and identification, resulting in the first fruit proteome of plantain species available until now. In addition we compared the results with the proteome from the dessert banana Cavendish (AAA). We identify in total 2390 proteins with false discovery rate of 0.2%. Genetically, both the dessert banana and the plantain are quite close but show a quite contrasting phenotype as mentioned above. The phenotype is undoubtedly associated to unique alleles. Using our in-house workflow for identifying allele specific peptides, we have found 98 candidate peptides to be unique for B genome and so encoded by a variety specific allele. From the 98 candidates we could confirm 59 mutations identified in the B genome. GO enrichment analysis of those B allele specific proteins showed that 20% of the proteins are related to sugar and/or starch metabolism. We hypothesize that the expression of B genome in plantain can be a key for the particular maturation process in plantain.
HostingRepository	PRIDE
AnnounceDate	2018-01-22
AnnouncementXML	Submission_2018-01-22_03:45:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nadia Campos
SpeciesList	scientific name: Musa; NCBI TaxID: 4640;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-16 08:33:58	ID requested
⏵ 1	2018-01-22 03:45:50	announced

Campos NA, Swennen R, Carpentier SC, The Plantain Proteome, a Focus on Allele Specific Proteins Obtained from Plantain Fruits. Proteomics, 18(3-4):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Banana, proteomic, plantain, banana breeding, allele specific

Sebastien Carpentier
contact affiliation	SYBIOMA: Facility for SYstems BIOlogy based MAss spectrometry, Leuven, Belgium Department of Biosystems, KULeuven, Willem Decroylaan 42 bus 2455- 3001 Leuven, Belgium. Tel. +32(0)16-379311
contact email	sebastien.carpentier@biw.kuleuven.be
lab head
Nadia Campos
contact affiliation	KULeuven
contact email	nadialvesnep@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005589
     1. Label: PRIDE project
     2. Name: Dessert banana vs plantain: characterization of allele specific protein isoforms that are correlated to the fruit maturation process