PXD005585

PXD005585 is an original dataset announced via ProteomeXchange.

Title	GFPtrap enrichment of CYP83A1 and CYP83B1
Description	CYP83A1 and CYP83B1 are important enzymes in the biosynthesis of glucosinolates. However, only few of their interaction partners are known so far. We therefore performed a GFPtrap affinity enrichment using protein extracts from lines expressing tagged CYP83A1 or CYP83B1, or from wild type plants as background control.
HostingRepository	PRIDE
AnnounceDate	2017-11-30
AnnouncementXML	Submission_2017-12-18_01:15:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005585
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katja Baerenfaller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); N-acetylated residue; oxidation; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-16 03:36:17	ID requested
1	2017-11-30 01:54:42	announced
⏵ 2	2017-12-18 01:15:29	announced	Updated publication reference for PubMed record(s): 29238354.

Nintemann SJ, Vik D, Svozil J, Bak M, Baerenfaller K, Burow M, Halkier BA, Unravelling Protein-Protein Interaction Networks Linked to Aliphatic and Indole Glucosinolate Biosynthetic Pathways in Arabidopsis. Front Plant Sci, 8():2028(2017) [pubmed]

curator keyword: Biological

submitter keyword: affinity enrichment, GFPtrap, glucosinolate biosynthesis, CYP83A1, CYP83B1, Arabidopsis, seedling

Katja Baerenfaller
contact affiliation	ETH Zurich
contact email	bkatja@ethz.ch
lab head
Katja Baerenfaller
contact affiliation	Institute of Plant Science
contact email	bkatja@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005585

PRIDE project URI

• PRIDE
  1. PXD005585
     1. Label: PRIDE project
     2. Name: GFPtrap enrichment of CYP83A1 and CYP83B1