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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-02-07
  • AnnouncementXML: Submission_2019-02-12_08:14:46.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Duc Duong
  • Title: Identifying the Ubiquitination Targets of E6AP by Orthogonal Ubiquitin Transfer
  • Description: E3 ubiquitin (UB) ligases, the ending module of the E1-E2-E3 cascades, transmit diverse signals in the cell by attaching UB to cellular proteins. Identifying E3 substrates holds the key to elucidate the roles of E3s in cell regulation. We constructed an orthogonal UB transfer (OUT) cascade to identify the substrates of E6AP, a HECT E3 also known as Ube3a that is implicated in neurodevelopmental disorders and cancer. We used yeast cell surface display to engineer E6AP to exclusively transfer an affinity-tagged UB variant (xUB) to its substrate proteins. Proteomic identification of xUB-conjugated proteins in HEK293 cells afforded 131 potential E6AP targets. Among them we verified MAPK1, CDK1 and CDK4, and PRMT5 are directly ubiquitinated by E6AP in vitro and in the cell. Our work establishes OUT as an efficient platform to profile E6AP substrates and would guide the engineering of OUT cascades with other E3s to interrogate their biological functions.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: Orbitrap Fusion

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-12-16 02:16:30ID requested
12018-02-09 11:45:26announced
22019-02-12 08:14:48announcedUpdated publication reference for PubMed record(s): 29263404.

Publication List

  1. Wang Y, Liu X, Zhou L, Duong D, Bhuripanyo K, Zhao B, Zhou H, Liu R, Bi Y, Kiyokawa H, Yin J, Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer. Nat Commun, 8(1):2232(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Ubiquitin, LC-MS/MS, IAP, conjugate

Contact List

    Jun Yin
    • contact affiliation: Georgia State University
    • contact email: junyin@gsu.edu
    • lab head:
    Duc Duong
    • contact affiliation: Center of Neurodegenerative Diseases
    • contact email: dduong@emory.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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