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PXD005584

PXD005584 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentifying the Ubiquitination Targets of E6AP by Orthogonal Ubiquitin Transfer
DescriptionE3 ubiquitin (UB) ligases, the ending module of the E1-E2-E3 cascades, transmit diverse signals in the cell by attaching UB to cellular proteins. Identifying E3 substrates holds the key to elucidate the roles of E3s in cell regulation. We constructed an orthogonal UB transfer (OUT) cascade to identify the substrates of E6AP, a HECT E3 also known as Ube3a that is implicated in neurodevelopmental disorders and cancer. We used yeast cell surface display to engineer E6AP to exclusively transfer an affinity-tagged UB variant (xUB) to its substrate proteins. Proteomic identification of xUB-conjugated proteins in HEK293 cells afforded 131 potential E6AP targets. Among them we verified MAPK1, CDK1 and CDK4, and PRMT5 are directly ubiquitinated by E6AP in vitro and in the cell. Our work establishes OUT as an efficient platform to profile E6AP substrates and would guide the engineering of OUT cascades with other E3s to interrogate their biological functions.
HostingRepositoryPRIDE
AnnounceDate2018-02-07
AnnouncementXMLSubmission_2019-02-12_08:14:46.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDuc Duong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-12-16 02:16:30ID requested
12018-02-09 11:45:26announced
22019-02-12 08:14:48announcedUpdated publication reference for PubMed record(s): 29263404.
Publication List
Wang Y, Liu X, Zhou L, Duong D, Bhuripanyo K, Zhao B, Zhou H, Liu R, Bi Y, Kiyokawa H, Yin J, Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer. Nat Commun, 8(1):2232(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Ubiquitin, LC-MS/MS, IAP, conjugate
Contact List
Jun Yin
contact affiliationGeorgia State University
contact emailjunyin@gsu.edu
lab head
Duc Duong
contact affiliationCenter of Neurodegenerative Diseases
contact emaildduong@emory.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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