PXD005583

PXD005583 is an original dataset announced via ProteomeXchange.

Title	N-terminome profiling in HEK293T
Description	Various forms of the protein, called proteoform, are generated by co- or post-translational modification, alternative splicing and alternative translation initiation site, resulting in interactions between ribosomes, mRNAs, tRNAs, and various enzymes. Here, we introduce a N-terminal peptide enrichment method (Nrich) using a negative selection on filter for the N-terminal peptides with two chemical reagents for labeling free amine and two endoproteases. We identified 6,525 acetylated (or partially acetylated) and 6,570 free protein N-termini from 5,727 proteins. The protein N-termini can be classified into nine groups, such as initial methionine removed or retained, non-terminal residue, signal/transit/pro-peptide removal, putative alternative translational initiation site (methionine removed or retained) and unknown processing, suggesting various proteoform in vivo. In addition, novel protein N-termini was identified in 5`-UTR sequence with pseudo start codon using customized database. Nrich can obtain information about protein stability, localization and function through the observation of finished N-terminal sequence of mature protein.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:03.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeonghun Yeom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-16 02:00:02	ID requested
1	2017-08-17 02:28:31	announced
⏵ 2	2024-10-22 04:35:08	announced	2024-10-22: Updated project metadata.

Yeom J, Ju S, Choi Y, Paek E, Lee C, Comprehensive analysis of human protein N-termini enables assessment of various protein forms. Sci Rep, 7(1):6599(2017) [pubmed]

10.1038/s41598-017-06314-9;

submitter keyword: protein stability, alternative translational initiation site, acetylation,N-terminome, positional proteomics, proteoform, N-termini, FASP, N-end rule, translational initiation site

Cheolju Lee
contact affiliation	Center for Theragnosis, Korea Institute of Science and Technology, Republic of Korea
contact email	clee270@kist.re.kr
lab head
Jeonghun Yeom
contact affiliation	prometabio co., ltd.
contact email	nature8309@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005583

PRIDE project URI

• PRIDE
  1. PXD005583
     1. Label: PRIDE project
     2. Name: N-terminome profiling in HEK293T