PXD005581 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tracking the embryonic stem cell transition from ground state pluripotency |
Description | Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life Rex1::GFP reporter to isolate cells either side of exit from naive status. Differentiation of Rex1-GFPd2 ES cells was initiated by withdrawing 2i (Kalkan et al., 2016). Undifferentiated 2i-cells and post-2i withdrawal differentiating populations (16h, 25h-Rex1-High, 25h-Rex1-Low) were subjected to proteomic analysis by Mass Spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2017-02-22 |
AnnouncementXML | Submission_2017-02-22_09:23:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kathryn Lilley |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-12-16 01:32:34 | ID requested | |
⏵ 1 | 2017-02-22 09:23:40 | announced | |
Publication List
Kalkan T, Olova N, Roode M, Mulas C, Lee HJ, Nett I, Marks H, Walker R, Stunnenberg HG, Lilley KS, Nichols J, Reik W, Bertone P, Smith A, Tracking the embryonic stem cell transition from ground state pluripotency. Development, 144(7):1221-1234(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Rex1, ES cell, epiblast, pluripotency |
Contact List
Kathryn Lilley |
contact affiliation | Department of Biochemistry, University of Cambridge |
contact email | ksl23@cam.ac.uk |
lab head | |
Kathryn Lilley |
contact affiliation | University of Cambridge |
contact email | ksl23@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005581
- Label: PRIDE project
- Name: Tracking the embryonic stem cell transition from ground state pluripotency