<<< Full experiment listing

PXD005581

PXD005581 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTracking the embryonic stem cell transition from ground state pluripotency
DescriptionMouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life Rex1::GFP reporter to isolate cells either side of exit from naive status. Differentiation of Rex1-GFPd2 ES cells was initiated by withdrawing 2i (Kalkan et al., 2016). Undifferentiated 2i-cells and post-2i withdrawal differentiating populations (16h, 25h-Rex1-High, 25h-Rex1-Low) were subjected to proteomic analysis by Mass Spectrometry.
HostingRepositoryPRIDE
AnnounceDate2017-02-22
AnnouncementXMLSubmission_2017-02-22_09:23:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKathryn Lilley
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-12-16 01:32:34ID requested
12017-02-22 09:23:40announced
Publication List
Kalkan T, Olova N, Roode M, Mulas C, Lee HJ, Nett I, Marks H, Walker R, Stunnenberg HG, Lilley KS, Nichols J, Reik W, Bertone P, Smith A, Tracking the embryonic stem cell transition from ground state pluripotency. Development, 144(7):1221-1234(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Rex1, ES cell, epiblast, pluripotency
Contact List
Kathryn Lilley
contact affiliationDepartment of Biochemistry, University of Cambridge
contact emailksl23@cam.ac.uk
lab head
Kathryn Lilley
contact affiliationUniversity of Cambridge
contact emailksl23@cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005581
PRIDE project URI
Repository Record List
[ + ]