PXD005580

PXD005580 is an original dataset announced via ProteomeXchange.

Title	Proteome dynamcis in biobanked horse peripheral blood derived lymphocytes (PBL) with induced autoimmune uveitis
Description	Equine recurrent uveitis (ERU) is the only spontaneous model for recurrent autoimmune uveitis in humans, where T cells target retinal proteins. Differences between normal and autoaggressive lymphocytes were identified in this study by analyzing peripheral blood derived lymphocytes (PBL) proteomes from the same case with IRBP-induced uveitis sampled before (day 0), during (day 15) and after uveitic attack (day 23). Relative protein abundances of PBL were investigated in a quantitative, label-free differential proteome analysis in cells that were kept frozen for 14 years since the initial experiment. Quantitative data could be acquired for 2632 proteins at all three time-points. Profound changes (> 2 fold change) in PBL protein abundance were observed when comparing day 0 to 15, representing acute inflammation (234 regulated proteins) and day 0 to 23 (cessation; 382 regulated). Significant differences applied to proteins with functions in integrin signaling during active uveitis, involving “Erk and pi-3 kinase are necessary for collagen binding in corneal epithelia”, “Integrins in angiogenesis” and “Integrin-linked kinase signaling” pathways. In contrast, at cessation of uveitic attack, significantly changed proteins belonged to pathways of “nongenotropic androgen signaling”, “classical complement pathway” and “Amb2 integrin signaling”. Several members of respective pathways were earlier shown to be changed in naturally occurring uveitis, underscoring the significance of these findings here and proofing the value of the induced model in mimicking spontaneous autoimmune uveitis.
HostingRepository	PRIDE
AnnounceDate	2017-08-31
AnnouncementXML	Submission_2017-08-31_00:07:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005580
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marlen Lepper
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-16 01:30:13	ID requested
⏵ 1	2017-08-31 00:07:20	announced

Hauck SM, Lepper MF, Hertl M, Sekundo W, Deeg CA, Proteome Dynamics in Biobanked Horse Peripheral Blood Derived Lymphocytes (PBL) with Induced Autoimmune Uveitis. Proteomics, 17(19):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Lymphocytes, autoimmune, Inflammation, CFA, frozen, LC-MSMS

Stefanie M. Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich, Research Center for Environmental Health (GmbH), Neuherberg,Germany
contact email	hauck@helmholtz-muenchen.de
lab head
Marlen Lepper
contact affiliation	Research Unit Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH)
contact email	marlen.lepper@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005580
     1. Label: PRIDE project
     2. Name: Proteome dynamcis in biobanked horse peripheral blood derived lymphocytes (PBL) with induced autoimmune uveitis