PXD005578

PXD005578 is an original dataset announced via ProteomeXchange.

Title	Chickpea Heat stress LC-MS - proteome data analysis
Description	Understanding the molecular differences in plant genotypes contrasting for heat sensitivity can provide useful insights into the mechanisms that confer heat tolerance in plants. This study is focused on comparative physiological and proteomic analyses of heat sensitive (ICC16374) and tolerant (JG14) genotypes of chickpea (Cicer arietinum L.) when subjected to heat stress at anthesis.Comparative gel-free proteome profiles indicated differences in the expression levels and regulation of common proteins that are associated with heat tolerance in contrasting genotypes under heat stress. The differentially regulated proteins were grouped into three categories based on their involvement in the molecular functions, cellular location and biological processes. Besides the identification of heat shock proteins, other proteins such as acetyl-CoA carboxylase, pyrroline-5-carboxylate synthase (P5CS), ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo), phenylalanine ammonia-lyase (PAL) 2, ATP synthase, glycosyltransferase, sucrose synthase and late embryogenesis abundant (LEA) proteins were strongly associated with heat tolerance in chickpea. Several crucial proteins such as cystathionine gamma-synthase, glucose-1-phosphate adenyltransferas, malate dehydrogenase, threonine synthase, and non-cyanogenic ß-glucosidase were induced by heat only in the heat tolerant genotype. Based on pathway analysis, we propose that proteins which are essentially related to the electron transport chain in photosynthesis, aminoacid biosynthesis, ribosome synthesis and secondary metabolite synthesis may play key roles in inducing tolerance to heat stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:48:26.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	santisree Parankusam
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-16 00:25:04	ID requested
1	2018-01-08 01:14:53	announced
2	2018-05-23 01:46:51	announced	Updated publication reference for DOI(s): 10.1016/j.envexpbot.2017.07.007.
⏵ 3	2023-11-14 06:48:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: QUANTITIAVE PROTEOMICS, CHICKPEA,HEAT STRESS, LC-MS

Santisree Parankusam
contact affiliation	International crops research institute for the semi-arid tropics
contact email	santhikinnu@gmail.com
lab head
santisree Parankusam
contact affiliation	ICRISAT
contact email	santhikinnu@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005578
     1. Label: PRIDE project
     2. Name: Chickpea Heat stress LC-MS - proteome data analysis