PXD005568

PXD005568 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals large amounts of decomposition enzymes and major metabolic pathways involved in algicidal process of Trametes versicolor F21a
Description	A recent algicidal mode indicates that fungal mycelia can wrap and eliminate almost all the co-cultivated algal cells within a short time. However, the regulation of molecular mechanism is rarely understood. Here, proteomic analysis was applied to investigate the algicidal process of Trametes versicolor F21a. Our results showed that 3,754 fungal proteins were identified, among which 2,809 unique proteins could be quantified during the process. 30 isoenzymes with the capacity of degradation biomass, belonging to Glycoside Hydrolases, Auxiliary Activities, Carbohydrate Esterases and Polysaccharide Lyases, were significantly up-regulated, suggesting that these enzymes probably employed synergistic mechanisms in degrading algal cells. Additionally, peptidase, exonuclease, manganese peroxidase and cytochrome c peroxidase were also up-regulated. 10% of the significantly up-regulated proteins were extracellular enzymes. Gene Ontology (GO) and KEGG pathway enrichment analysis demonstrated that the enriched metabolic pathways mainly contained carbon metabolism, selenocompound metabolism, sulfur assimilation and metabolism, as well as several amino acid biosynthesis pathways, which implied that these pathways should play vital roles in the synthesis of needed nutrition for the fungal mycelia via components of algal cells. Moreover, the fungal NmrA-like transcriptional regulator which represses the nitrogen metabolite was also enriched and might be a key regulator in eliminating algal cells
HostingRepository	PRIDE
AnnounceDate	2018-04-13
AnnouncementXML	Submission_2018-04-13_07:24:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005568
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	zhu taotao
SpeciesList	scientific name: Trametes versicolor; NCBI TaxID: 5325;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-14 03:32:12	ID requested
1	2018-04-13 00:20:41	announced
⏵ 2	2018-04-13 07:24:25	announced	Updated publication reference for PubMed record(s): 28634367.

Gao X, Wang C, Dai W, Ren S, Tao F, He X, Han G, Wang W, Proteomic analysis reveals large amounts of decomposition enzymes and major metabolic pathways involved in algicidal process of Trametes versicolor F21a. Sci Rep, 7(1):3907(2017) [pubmed]

curator keyword: Biological

submitter keyword: Trametes versicolor F21a, LC-MS/MS

Guomin Han
contact affiliation	School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
contact email	guominhan@ahau.edu.cn
lab head
zhu taotao
contact affiliation	PTM biolab (Hangzhou)
contact email	taotao_zhu@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005568
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals large amounts of decomposition enzymes and major metabolic pathways involved in algicidal process of Trametes versicolor F21a