PXD005567

PXD005567 is an original dataset announced via ProteomeXchange.

Title	Kv7.2 LC-MSMS - Updating in vivo and in vitro phosphorylation and methylation sites of voltage-gated Kv7
Description	Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of Kv7 channels is regulated by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signalling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2), Ca2+/Calmodulin and phosphorylation. Recent studies have reported that PIP2 sensitivity of Kv7.2 channels is affected by two PTMs, phosphorylation and methylation, harboured in the PIP2 binding domains. Here this project aimed to update phosphorylation and methylation sites on Kv7.2 from heterologous cells, the rat brain and GST-fusion Kv7.2 N- and C-terminal ends proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:03.474.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005567
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jae-Won Yang
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Methyl; Oxidation; Dimethyl
Instrument	Bruker Daltonics micrOTOF series; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-14 03:27:27	ID requested
1	2017-08-25 02:36:50	announced
⏵ 2	2024-10-22 04:39:08	announced	2024-10-22: Updated project metadata.

Erdem FA, Salzer I, Heo S, Chen WQ, Jung G, Lubec G, Boehm S, Yang JW, Updating In Vivo and In Vitro Phosphorylation and Methylation Sites of Voltage-Gated Kv7.2 Potassium Channels. Proteomics, 17(19):(2017) [pubmed]

10.1002/pmic.201700015;

10.6019/PXD005567;

curator keyword: Biological

submitter keyword: methylation,Kv7.2, phosphorylation, KCNQ2

Jae-Won Yang
contact affiliation	Institute of Pharmacology Center for Physiology and Pharmacology Medical University of Vienna, Austria
contact email	jae-won.yang@meduniwien.ac.at
lab head
Jae-Won Yang
contact affiliation	Medical University of Vienna
contact email	jae-won.yang@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005567

PRIDE project URI

• PRIDE
  1. PXD005567
     1. Label: PRIDE project
     2. Name: Kv7.2 LC-MSMS - Updating in vivo and in vitro phosphorylation and methylation sites of voltage-gated Kv7