PXD005551

PXD005551 is an original dataset announced via ProteomeXchange.

Title	Rho-kinase inhibitor Y-27632 and hypoxia synergistically enhance chondrocytic phenotype and change the S100 protein profile in human chondrosarcoma cells
Description	Different kinds of cell-based therapies and tissue engineering applications need in vitro cultured differentiated chondrocytes. However, articular chondrocytes are slowly dividing cells, which in culture tend to lose their cell type-specific phenotype and ability to produce structurally and functionally correct cartilage tissue. Here we show that Y-27632 treatment at hypoxia enhances efficiently chondrocyte-specific extracellular matrix production on chondrocytic cells. The effects of the long-term Y-27632 exposure to human chondrosarcoma 2/8 cell phenotype maintenance and extracellular matrix production were studied at normoxia and at 5% low oxygen atmosphere. The Y-27632 treatment at normoxia induced ACAN and COL2A1 gene up-regulation and minor increase of sulfated glycosaminoglycans (sGAGs), while type II collagen expression was not significantly up-regulated. The most efficient expression of ACAN and COL2A1 was achieved when the Y-27632 treatment was performed at hypoxia. The production of sGAGs increased by 65.8%, and the ELISA analysis revealed over 6 times up-regulation on type II collagen expression. The Y-27632 induced up-regulation of S100-A1 and S100-B proteins, and also affected the expression of several other S100 protein family members, such as S100-A4, S100-A6, S100-A13 and S100-A16. The up-regulation of S100-A1 and S100-B proteins is suggested to enhance the chondrocytic phenotype of the cells.
HostingRepository	PRIDE
AnnounceDate	2017-06-22
AnnouncementXML	Submission_2017-06-22_00:05:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joakim Bygdell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-12 05:44:37	ID requested
⏵ 1	2017-06-22 00:05:53	announced

Piltti J, Bygdell J, Fern, á, ndez-Echevarr, í, a C, Marcellino D, Lammi MJ, Rho-kinase inhibitor Y-27632 and hypoxia synergistically enhance chondrocytic phenotype and modify S100 protein profiles in human chondrosarcoma cells. Sci Rep, 7(1):3708(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, LC/MS, chondrosarcoma

Mikko Lammi
contact affiliation	Departement of Integrative Medical Biology, Umeå University, SWEDEN
contact email	mikko.lammi@umu.se
lab head
Joakim Bygdell
contact affiliation	Computational Life Science Cluster (CLiC), Deptartment of Chemistry, Umeå University
contact email	joakim.bygdell@umu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005551
     1. Label: PRIDE project
     2. Name: Rho-kinase inhibitor Y-27632 and hypoxia synergistically enhance chondrocytic phenotype and change the S100 protein profile in human chondrosarcoma cells