PXD005548 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular and cellular profile of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica |
Description | The liver fluke Fasciola hepatica is an economically important pathogen of livestock. Fasciolosis in humans is an important re-emerging zoonosis, with 180 million people at risk. Development of novel control strategies requires an understanding of parasite virulence and tissue invasion, and of how the parasite evades and modulates the immune response during early infection. A combination of immunological and proteomic analyses was employed to investigate the peritoneal fluid of sheep infected with F. hepatica to characterise early tissue invasion and liver pathogenesis. At 18 days post-infection (dpi), histopathology of sheep liver showed white necrotic foci/tracts indicative of F. hepatica migration. Within the peritoneal fluid of infected animals, specific F. hepatica antigen FhCL1 antibodies coincided with an intense innate and adaptive cellular immune response, with increasing numbers of total leukocytes and a marked eosinophilia (49%). Cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated but not statistically significant at 18 dpi compared to uninfected sheep indicating that whilst the immune response is developing it has not polarised to the Th2 type associated with chronic fasciolosis. Proteomic analysis of the peritoneal fluid identified 178 proteins, with 25 proteins more highly expressed in the infected animals, based on fold changes in protein concentration compared to the uninfected animals. Components of the liver extracellular matrix, including collagen, periostin and the adhesion protein, VCAM-1, exhibited increased expression associated with early fasciolosis, which may be important in signalling host immune responses to tissue damage. In early F. hepatica infection cellular infiltration with marked eosinophilia, adaptive immune responses and products of liver pathology are evident in the peritoneum. Although cytokine responses are developing they are mixed and not yet polarised to a Th2-type. We have characterised biomarkers of parasite-induced liver damage which could be exploited for diagnosis, and vaccine development aimed at reducing/preventing liver pathology. |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-26 |
AnnouncementXML | Submission_2018-03-26_06:50:56.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005548 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Krystyna Cwiklinski |
SpeciesList | scientific name: Ovis aries (Sheep); NCBI TaxID: 9940; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-12-12 02:27:38 | ID requested | |
⏵ 1 | 2018-03-26 06:50:57 | announced | |
Publication List
Ruiz-Campillo MT, Molina Hernandez V, Escamilla A, Stevenson M, Perez J, Martinez-Moreno A, Donnelly S, Dalton JP, Cwiklinski K, Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica. Sci Rep, 7(1):2782(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Fasciola hepatica |
liver fluke |
acute infection |
immunopathology |
Contact List
John P Dalton |
contact affiliation | School of Biological Sciences, Queen's University Belfast Institute for Global Food Security, Queen's University Belfast |
contact email | j.dalton@qub.ac.uk |
lab head | |
Krystyna Cwiklinski |
contact affiliation | Queen's University Belfast |
contact email | k.cwiklinski@qub.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD005548 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005548
- Label: PRIDE project
- Name: Molecular and cellular profile of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica