PXD005533

PXD005533 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of seminal plasma from alternative reproductive tactics of Chinook salmon (Oncorhynchus tswatchysha)
Description	A spectrum of reproductive strategies can manifest within the same species to help improve reproductive advantage when faced under the pressure of sexual competition, known as alternate reproductive tactics (ARTs). Chinook salmon are external fertilizing species that exhibit the sneak-guard strategy of ARTs. The larger hooknose males dominate for optimal mating position, while jack males rely on the sneak tactic to achieve fertilization in relation to their smaller body size. During copulation, a single female’s egg may be fertilized by more than one male. Therefore the threat of sperm competition is increased as the ejaculate from multiple males can interact during this time. As jack males establish a position further away from the female’s egg during fertilization attempts, it is proposed that jacks should invest more in spermatozoa quality. Although poorly understood, previous studies have suggested that differences in spermatozoa quality may play a critical role in sperm competition. Considering that the seminal plasma strongly regulates spermatozoa quality and other processes critical for fertilization success, we employed label free quantitative mass spectrometry utilizing ion mobility separation coupled to cross-species bioinformatics to examine the seminal plasma proteome of Chinook salmon.
HostingRepository	PRIDE
AnnounceDate	2025-11-28
AnnouncementXML	Submission_2025-11-28_01:41:47.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Gombar
SpeciesList	scientific name: Oncorhynchus tshawytscha; NCBI TaxID: NEWT:74940;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-09 02:40:27	ID requested
⏵ 1	2025-11-28 01:41:48	announced

Gombar R, Pitcher TE, Lewis JA, Auld J, Vacratsis PO, Proteomic characterization of seminal plasma from alternative reproductive tactics of Chinook salmon (Oncorhynchus tswatchysha). J Proteomics, 157():1-9(2017) [pubmed]

10.1016/j.jprot.2017.01.019;

curator keyword: Biological

submitter keyword: data independent acquisition, proteomics, sperm competition,Seminal plasma, label-free quantitation, ion mobility

Panayiotis O. Vacratsis
contact affiliation	Chemistry and Biochemistry, University of Windsor, Canada
contact email	vacratsi@uwindsor.ca
lab head
Robert Gombar
contact affiliation	University of Windsor
contact email	gombarr@uwindsor.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD005533

PRIDE project URI

• PRIDE
  1. PXD005533
     1. Label: PRIDE project
     2. Name: Proteomic characterization of seminal plasma from alternative reproductive tactics of Chinook salmon (Oncorhynchus tswatchysha)