PXD005527

PXD005527 is an original dataset announced via ProteomeXchange.

Title	Unbiased proteomic study of the axons of cultured rat cortical neurons
Description	We have conducted quantitative proteomic analyses of the axons of cultured rat cortical neurons. Axons are isolated by using glass chips that enable the axons and their cell bodies of neurons to grow in separated regions on the chip surface. Proteins extracted from the isolated axons, as well as those extracted from whole cortical neurons are subjected to two-dimensional liquid chromatography (2D-LC)-mass spectrometry (MS)-MS analyses. The abundances of proteins in the axon are found to be strongly correlated with their average abundances in whole neurons. Based upon these data, a quantitative description of the protein distribution among various subcellular structures in the axon has been generated. The proteins extracted from the axons and whole neurons are also subjected to stable isotope dimethyl labeling reaction and then to 2D-LC-MS/MS analysis. Proteins enriched in the axon compartment of rat cortical neurons are thus identified.
HostingRepository	PRIDE
AnnounceDate	2018-04-17
AnnouncementXML	Submission_2018-04-17_00:27:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chih-Fan Chuang
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-08 01:58:45	ID requested
⏵ 1	2018-04-17 00:27:21	announced

Chuang CF, King CE, Ho BW, Chien KY, Chang YC, Unbiased Proteomic Study of the Axons of Cultured Rat Cortical Neurons. J Proteome Res, 17(5):1953-1966(2018) [pubmed]

curator keyword: Biological

submitter keyword: Rat, 2D-LC-MSMS, Axon, Label-free quantification, Dimethyl-labeling quantitative proteomics

Yen-Chung Chang
contact affiliation	Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu, Taiwan, Republic of China
contact email	ycchang@life.nthu.edu.tw
lab head
Chih-Fan Chuang
contact affiliation	Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, Republic of China
contact email	ion516@yahoo.com.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005527
     1. Label: PRIDE project
     2. Name: Unbiased proteomic study of the axons of cultured rat cortical neurons