PXD005517

PXD005517 is an original dataset announced via ProteomeXchange.

Title	Phylointeractomics reconstructs functional evolution of protein binding
Description	Molecular phylogenomics investigates evolutionary relationships based on genomic data. However, despite genomic sequence conservation, changes in protein interactions can occur relatively rapidly and may cause strong functional diversification. To investigate such functional evolution, we here combine phylogenomics with interaction proteomics. We develop this concept by investigating the molecular evolution of the shelterin complex, which protects telomeres, across 16 vertebrate species from zebrafish to humans covering 450 million years of evolution. Our phylointeractomics screen discovers previously unknown telomere-associated proteins and reveals how homologous proteins undergo functional evolution. For instance, we show that TERF1 evolved as a telomere-binding protein in the common stem lineage of marsupial and placental mammals. Phylointeractomics is a versatile and scalable approach to investigate evolutionary changes in protein function and thus can provide experimental evidence for phylogenomic relationships.
HostingRepository	PRIDE
AnnounceDate	2017-02-15
AnnouncementXML	Submission_2017-02-15_10:38:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Taeniopygia guttata (Zebra finch) (Poephila guttata); NCBI TaxID: 59729;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-07 02:06:55	ID requested
⏵ 1	2017-02-15 10:38:55	announced

Kappei D, Scheibe M, Paszkowski-Rogacz M, Bluhm A, Gossmann TI, Dietz S, Dejung M, Herlyn H, Buchholz F, Mann M, Butter F, Phylointeractomics reconstructs functional evolution of protein binding. Nat Commun, 8():14334(2017) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, interactomics, telosome

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Dept. of Proteomics and Signal Transduction
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005517

PRIDE project URI

• PRIDE
  1. PXD005517
     1. Label: PRIDE project
     2. Name: Phylointeractomics reconstructs functional evolution of protein binding